Genetic and antibiotic resistance profiles of thermophilic Campylobacter spp. isolated from quails (Coturnix coturnix japonica) in a Portuguese slaughterhouse |
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| Affiliation: | 1. College of Construction Engineering, Jilin University, Changchun 130026, China;2. Ministry of Education Key Laboratory of Groundwater Resources and Environment, Jilin University, Changchun 130000, China;1. Institute for Multi-disciplinary Convergence of Matter, Korea Institute of Science and Technology, Seoul 130-650, Republic of Korea;2. Department of Physics, Aliah University, DD – 45, Sector – I, Salt Lake, Kolkata 700064, India;3. Engineering Division, Brown University, Providence, RI 02912, USA;1. Kushlan Tropical Biology Institute, Fairchild Tropical Botanic Garden, Coral Gables, Miami, FL 33156, USA;2. Jardín Botánico Nacional, Avenida República de Argentina, Santo Domingo, Dominican Republic;3. Jardin Botanique des Cayes, Bergeaud, Route National 2, Les Cayes, Haiti;4. USDA-ARS-SHRS, National Germplasm Repository, Miami, FL 33158, USA;5. Montgomery Botanical Center, Coral Gables, Miami, FL 33156, USA;6. International Center for Tropical Botany, Kimberly Green Latin American and Caribbean Center, Cuban Research Institute, Florida International University, Miami, FL 33199, USA;7. Department of Biological Sciences, Florida International University, Miami, FL 33199, USA;1. Instituto Nacional de Investigação Agrária e Veterinária, I.P. (INIAV, IP), Rua General Morais Sarmento, s/n, 1500-310 Lisboa, Portugal;2. Universidade de Lisboa, Faculdade de Ciências, Biosystems and Integrative Sciences Institute (BioISI), Edificio TecLabs, Campus da FCUL, Campo Grande, 1749-016 Lisboa, Portugal;1. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, PR China;2. University of Chinese Academy of Sciences, Beijing, 100049, PR China;3. Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, PR China;1. Department of Microbiology, Faculty of Veterinary Medicine, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran;2. Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran;3. Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran |
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| Abstract: | The aim of our study was to evaluate thermophilic Campylobacter frequency in quails at slaughterhouse level in two subsequent years. Campylobacter isolates were evaluated for their antibiotics resistance ability, looking for multidrug resistance. Their genetic diversity assessment was performed by pulsed field gel electrophoresis (PFGE) in order to establish possible relationships among different flocks and producers. This study highlights the high frequency (81–94%) and antimicrobial resistance of Campylobacter spp. in commercial quails. Campylobacter coli were dominant with a high frequency of resistance for fluoroquinolones, tetracycline and ampicillin among all the quail flocks and producers. C. coli strains with the same genetic profile isolated in samples from different flocks and producers, and collected in different slaughtering days, suggest the existence of common risk factors at production level in a vertically integrated enterprise. Strains' low genetic diversity with the prevalence of a single pulsotype among producers, might suggest that quails have been submitted continuously to the same therapeutics, which induced selectivity due to antibiotic stress pressure. C. coli contaminated quails might act as a source that increases consumer's exposure to multidrug-resistant isolates. |
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| Keywords: | Poultry Quail Antimicrobial-resistance Multidrug resistance Pulsotype AMP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" Ampicillin (10 μg) ERI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" Erythromicin (15 μg) TET" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" Tetracycline (30 μg) CHL" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" Chloramphenicol (30 μg) GEN" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" Gentamicin (10 μg) CIP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" Ciprofloxacin (5 μg) NA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" Nalidixic acid (10 μg) NOR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0115" }," $$" :[{" #name" :" text" ," _" :" Norfloxacin (5 μg) OFX" },{" #name" :" keyword" ," $" :{" id" :" kwrd0125" }," $$" :[{" #name" :" text" ," _" :" Ofloxacin (5 μg) AUG" },{" #name" :" keyword" ," $" :{" id" :" kwrd0135" }," $$" :[{" #name" :" text" ," _" :" Amoxacillin + Clavulanic acid (20 + 10 μg) STX" },{" #name" :" keyword" ," $" :{" id" :" kwrd0145" }," $$" :[{" #name" :" text" ," _" :" Trimethoprim + Sulfamethoxazole (25 μg) |
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