双水相萃取分离葡萄籽中原花青素

采用双水相萃取葡萄籽中原花青素,首先研究双水相萃取体系组成、辅助萃取方法、萃取策略对原花青素萃取效果的影响,并采用正交试验对原花青素萃取条件进行优化。结果表明,双水相萃取体系组成为无水乙醇/硫酸铵,辅助萃取方法为微波辅助法,萃取策略为乙醇+水+葡萄籽微波处理后加入硫酸铵,条件为无水乙醇/硫酸铵萃取体系为质量分数30%无水乙醇/质量分数20%硫酸铵,加入葡萄籽质量分数为3%,pH为4.0,微波功率100 W,微波时间5 min,在该条件下,葡萄籽中原花青素的萃取率为94.1%,得率为25.8mg/g;

Separation of procyanidin from grape seed by aqueous two-phase system

Procyanidin from grape seed were separated and purified by the aqueous phase two-extraction. Aqueous two-phase extraction systems, differently assisted extraction methods and the extraction strategies were firstly studied. The best extractions of procyanidin by aqueous two-phase system were investigated based on orthogonal experimental design. The results showed that, in the aqueous two-phase extraction system of ethanol and (NH₄)₂SO₄, the extraction method was the microwave-assisted extraction, i.e., Ethanol, water, and compounded by microwave treatment, as well as (NH₄)₂SO₄ were added. It was found that the best extraction conditions were 30%(w/w) ethanol/20%(w/w) (NH₄)₂SO₄, 3%(w/w) grape seed powders, pH=4.0, microwave power 100W, ,and microwave time 5 min. Under these best extraction conditions, the recovery rate of procyanidin was 94.1%, and its yield was 25.8 mg/g.