Structural changes of casein micelles in a rennin gradient film with simultaneous consideration of the film morphology期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Structural changes of casein micelles in a rennin gradient film with simultaneous consideration of the film morphology

Authors:	Ronald Gebhardt Stephan V. Roth Manfred Burghammer Christian Riekel Alexander Tolkach Ulrich Kulozik Peter Müller-Buschbaum

Affiliation:	1. European Synchrotron Radiation Facility, B.P. 220, F-38043 Grenoble Cedex, France;2. HASYLAB at Desy, Notkestr. 85, 22603 Hamburg, Germany;3. Technische Universität München, Chair for Food Process Engineering and Dairy Technology, Weihenstephaner Berg 1, 85354 Freising-Weihenstephan, Germany;4. Technische Universität München, Physik Department E13, James-Franck-Str. 1, 85747 Garching, Germany;5. ZIEL Center of Nutrition and Food Research, Technology Section, Technische Universität München, Weihenstephaner Berg 1, 85354 Freising-Weihenstephan, Germany;1. Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Vojvode Stepe 444a, Belgrade, Serbia;2. Faculty of Biology, University of Belgrade, Studentski trg 16, Belgrade, Serbia;3. Institute of Oncology and Radiology of Serbia, Pasterova 14, Belgrade, Serbia;1. Department of Mechanical Engineering and Materials Science, Duke University, Durham, NC 27708, USA;2. Department of Mathematics, Duke University, USA;1. Department of Electronic Science, University of Delhi South Campus, New Delhi 110023, India;2. Inter University Accelerator Centre, Aruna Asaf Ali Marg, New Delhi 110067, India;3. Department of Physics, Bhagini Nivedita College, Delhi University, Delhi 110043, India;1. Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche (STEBICEF), Università degli Studi di Palermo, Via Archirafi 32, 90123 Palermo, Italy;2. Istituto di Biomedicina ed Immunologia Molecolare (IBIM), CNR, Via Ugo La Malfa, 153, Palermo, Italy;3. Istituto per lo Studio dei Materiali Nanostrutturati (ISMN), CNR, Via Ugo La Malfa, 153, 90146 Palermo, Italy;1. Food for Health Ireland, University College Dublin, Dublin 4, Ireland;2. UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland;3. School of Medicine, University College Dublin, Dublin 4, Ireland;4. School of Biomolecular & Biomedical Science, University College Dublin, Dublin 4, Ireland;5. Mass Spectrometry Resource, University College Dublin, Dublin 4, Ireland

Abstract:	The effect of rennin on the micelle structure and microstructure of casein films was investigated with micro-beam grazing incidence small angle X-ray scattering and optical microscopy. Size distributions of casein micelles and colloidal calcium phosphate particles were determined as a function of rennin concentration within a rennin gradient film. While the size of the casein micelles decreased with increasing rennin concentration, the size of the colloidal calcium phosphate clusters remained constant. In accordance with a first-order enzyme reaction mechanism, the rennin-induced decay of the micellar sizes could be well described by an exponential function. Two distinct film morphologies at high and low rennin concentration were observed. At intermediate rennin concentration a two-phase surface structure was found, in which both film morphologies coexisted. The conclusions drawn on the behaviour of casein micelles in films can generally be applied to the rennin-induced changes in casein micelles in bulk solutions.

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