| 高效液相色谱法测定鱼油中的EPA乙酯和DHA乙酯 |
| |
| 引用本文： | 张怡评,晋文慧,陈伟珠,谢全灵,方华,洪专,易瑞灶.高效液相色谱法测定鱼油中的EPA乙酯和DHA乙酯[J].中国食品添加剂,2012(1):233-237. |
| |
| 作者姓名： | 张怡评 晋文慧 陈伟珠 谢全灵 方华 洪专 易瑞灶 |
| |
| 作者单位： | 1. 国家海洋局第三海洋研究所,厦门361005;南京中医药大学,南京210046
2. 国家海洋局第三海洋研究所,厦门,361005 |
| |
| 基金项目： | 海洋公益性行业科研专项经费项目(项目编号:200905022);福建省科技计划项目(项目编号:2009Y0035) |
| |
| 摘 要： | 目的:建立高效液相色谱法分离测定鱼油制品中的二十碳五烯酸乙酯(EPA-EE)和二十二碳六烯酸乙酯(DHA-EE)的方法。方法:采用C18色谱柱(250mm×4.6mm),以乙腈-水(90∶10)溶液为流动相,流速为1.0mL.min-1,检测波长205nm。结果:EPA-乙酯和DHA-乙酯分别在0.00273.40μg和0.0031 3.92μg的范围内呈良好线性,线性回归方程分别为y=46014x+25975(R2=1)和y=27036x+53635(R2=0.9995),EPA乙酯和DHA乙酯平均回收率分别为99.45%和101.78%,相对标准偏差分别为1.84%和2.92%(n=5),鱼油中EPA-乙酯和DHA乙酯的含量分别为8.15%和59.6%。结论:本法简便、快速、重现性好,可用于油脂样品中EPA、DHA乙酯含量测定。
|
| 关 键 词： | EPA DHA 鱼油 高效液相 |
Determination of EPA- EE and DHA- EE in fish oil by HPLC |
| |
| ZHANG Yi-ping
,
JIN Wen-hui
,
CHEN Wei-zhu
,
XIE Quan-ling
,
FANG Hua
,
HONG Zhuan
,
YI Rui-zao.Determination of EPA- EE and DHA- EE in fish oil by HPLC[J].China Food Additives,2012(1):233-237. |
| |
| Authors: | ZHANG Yi-ping JIN Wen-hui CHEN Wei-zhu XIE Quan-ling FANG Hua HONG Zhuan YI Rui-zao |
| |
| Affiliation: | 1(1.The 3rd Institute of Oceanography State Oceanic Administration,Xiamen 361005;2.Nanjing University of Chinese Medicine,Nanjing 210046) |
| |
| Abstract: | Objective:To establish a method for the determination of EPA-EE and DHA-EE in fish oil by high performance liquid chromatography(HPLC).Method:The determination was carried out on C18 column.The mobile phase consisted of acetonitrile water(90 ∶10)with flow rate of 1.0 mL/min and detection wavelength set at 205 nm.Results:The linear ranges were 0.00273.40g for EPA-EE(R2=1) and 0.00313.92μg for DHA-EE(R2=0.9995).The average recoveries were 99.45% for EPA-EE(RSD=1.84%)and 101.78% for DHA-EE(RSD=2.92%).Conclusion:The method is simpie,rapid,and reproducibie,it can be used for the detection of EPA-EE and DHA-EE in fish oil. |
| |
| Keywords: | EPA-EE DHA-EE Fish oil HPLC |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
