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| 食用精炼大豆油DNA提取及单管巢式PCR检测 | |
| 引用本文： | 姚芹, 宋浩, 陈枫. 食用精炼大豆油DNA提取及单管巢式PCR检测[J]. 食品工业科技, 2013, (21): 183-186. DOI: 10.13386/j.issn1002-0306.2013.21.027 |
| 作者姓名： | 姚芹 宋浩 陈枫 |
| 作者单位： | 1.苏州农业职业技术学院,江苏省食品安全快速检测工程技术研究开发中心;2.苏州市吴江区农业委员会;3.苏州市吴江区农产品检测中心 |
| 摘 要： | 设计了针对转基因Roundup Ready大豆外源基因扩增的内外2对引物,分别位于CaMV35s启动子,CTP基因,CP4EPSPS基因区域,并成功对精炼大豆油中的外源基因进行单管巢式PCR检测。结果表明,用改良试剂盒法和冷冻干燥法提取大豆油中的DNA均可以满足单管巢式PCR检测要求;单管巢式PCR扩增对内外引物的Tm值有特殊要求,外引物的退火温度高于内引物。本实验建立的精炼大豆油转基因成分的单管巢式PCR检测方法特异性强,灵敏度高且快速方便。 |
| 关 键 词： | 精炼大豆油 DNA提取 CP4EPSPS基因 单管巢式PCR |
| 收稿时间： | 2013-04-02 |
DNA extraction from soybean oil and detection of genetically-modified components by single-tube nested PCR |
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| YAO Qin, SONG Hao, CHEN Feng. DNA extraction from soybean oil and detection of genetically-modified components by single-tube nested PCR[J]. Science and Technology of Food Industry, 2013, (21): 183-186. DOI: 10.13386/j.issn1002-0306.2013.21.027 | |
| Authors: | YAO Qin SONG Hao CHEN Feng |
| Affiliation: | 1.Suzhou Polytechnic Institute of Agriculture;2.Agriculture Commission of Suzhou City Wujiang;3.Wujiang Agricultural Products Testing Center |
| Abstract: | The two pairs of primers were synthesized according to genetically- modified soybean, located in the CaMV35s promoter, CTP gene, CP4EPSPS gene region, and detection method of transgenic factors by single-tube nested PCR was established.It was proved that DNA extraction by improved reagent kit method or freeze drying technology from soybean oil could meet the detection of single-tube nested PCR, and for single-tube nested PCR outer primers annealing temperature must be higher than inner primers. |
| Keywords: | soybean oil DNA extraction CP4EPSPS gene single-tube nested PCR |
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