桑叶提取物抑菌活性及其成分分析

为了探讨桑叶提取物的抑菌活性及其物质基础,采用微量稀释法对桑叶70%乙醇提取物的4个萃取部位进行抑菌活性测试并利用气相色谱-质谱联用技术对抑菌活性强的萃取部位进行了成分分析,结果表明桑叶70%乙醇提取物4个萃取部位中石油醚部位抑菌活性最强,其次为乙酸乙酯部位,正丁醇部位和水部位。气相色谱-质谱检测分析石油醚萃取部位的主要成分及其质量分数为植物醇（24.87%）、环二十八烷（18.00%）、棕榈酸（10.20%）、α-亚麻酸（9.06%）、亚麻酸乙酯（7.92%）、硬脂酸（7.14%）,另外还含有少量α-生育酚和β-谷甾醇等活性成分。 

Antibacterial activity and component analysis of muberry (Morus alba L.) leaves extract

In order to explore the antimicrobial activity and material basis of mulberry leaves extract, the antibacterial activities of 4 fractions (petroleum ether, ethyl acetate, n-butanol and water) from muberry leaves crude extract were tested using micro-plate dilution assay.And the fraction of the strongest antibacterial activity was analyzed by gas chromatography-mass spectrometry.The results indicated that petroleum ether fraction had the strongest antibacterial activity, followed by ethyl acetate fraction, n-butanol fraction and water fraction.Then, the petroleum ether fraction was analyzed by gas chromatography-mass spectrometry.The result showed that this fraction was rich of phytol (24.87%) , cyclo octacosane (18.00%) , hexadecanoic acid (10.20%) , α-linolenic acid (9.06%) , ethyl linolenate (7.92%) , and octadecanoic acid (7.14%) .In addition, it also contained a small amount of α-tocopherol, β-sitosterol and other components.