一株墨鱼干源中度嗜盐菌的系统发育分析与生长特性

从市售墨鱼干表面分离纯化出一株中度嗜盐细菌NYJ01。16S rDNA序列测定和BLAST分析结果表明其16S rDNA与盐单胞菌属和栖盐田菌属中一些菌株的序列一致性高达99%。系统发育分析显示它与栖盐田菌属中4个种的模式菌株聚类在一个进化枝,表明菌株NYJ01是一种栖盐田菌。其耐盐范围是1%~20%NaCl,最适浓度为3%~10%NaCl;生长温度范围是10~45℃,最适温度为25~37℃;该菌在0.5%~15%的NaNO3培养液中生长良好,但不具有硝酸盐还原作用。可利用0.025%双乙酸钠、0.05%山梨酸钾、0.05%苯甲酸钠或0.05%脱氢乙酸钠等食品防腐剂抑制菌株NYJ01的生长。 

Phylogenetic analysis and growth characteristics of a moderate halophilic bacterium isolated from dried cuttlefish

A moderate halophilic bacterium was isolated from the surface of a commercial dried cuttlefish ( Sepia officinalis) product.The sequencing of the bacterial 16S rDNA and BLAST analysis suggested the fragment shares99% identity with that of some species of genera Halomonas and Salinicola.Phylogenetic analysis result showed the halophile and the four type strains of genus Salinicola clustered into a branch in the phylogenetic consensus tree.Therefore, the strain NYJ01 was a member of genus Salinicola, and a tentative name of Salinicola sp. NYJ01 was proposed.For the strain NYJ01, growth occurred at sodium chloride concentrations of 1% ²0% ( optimum at 3% ¹0%) , at 10~ 45℃ ( optimum at 25 ~ 37℃) . The strain grew well in mediums that consisted of 0.5% ~ 15% sodium nitrate, but it was not able to reduce nitrate into nitrite. Some food preservatives, e.g., 0.025% sodium diacetate, 0.05% potassium sorbate, 0.05% sodium benzoate or 0.05% sodium dehydroacetate can be used to inhibit growth of the strain NYJ01.