白首乌酒对癫痫大鼠神经元保护作用及其机制研究

为了优化苹果酵素自然发酵工艺,通过Box-Behnken试验设计,研究初始pH值、糖添加量、苹果片质量浓度对苹果酵素感官评分和超氧化物歧化酶（SOD）活性的影响,并采用超高效液相色谱（UPLC）及液相色谱-质谱联用技术（LC-MS）分析了酚类物质的组成和含量。结果表明,优化出自然发酵工艺条件为初始pH值4.6,糖添加量15%,苹果片含量200 g/L。在此优化条件下,苹果酵素感官评分为（8.45±0.24）分、SOD活性为（40.26±1.03） U/mL。苹果酵素共检测出15种酚类物质,其中绿原酸含量最高,原花青素B2、表儿茶素、原花青素B1、槲皮素-半乳糖苷、儿茶素以及根皮苷次之。

Research on the protective effect and mechanism of Cynanchum bungei liquor on neurons in epileptic rats

To investigate the protective effect and mechanism of Cynanchum bungei liquor on neurons in epileptic rats, 50 SD rats were randomly divided into blank group, model group, C. bungei liquor extract (CBLE) low-dose group (4 ml/kg) and high-dose group (10 ml/kg). Except for the blank group, the epileptic model was established in the other groups, and Racine score, epilepsia seizure frequency and first epilepsia seizure duration were performed after successful modeling. The damage of rat neurons was observed by hematoxylin-eosin (HE) staining and section scanning. The expression of p38MAPK protein in hippocampus of rat brain was analyzed by Western Bolt. The results shown that it was found that compared with the model group, the Racine score, epilepsia seizure frequency and first epilepsia seizure duration of each dose groups of CBLE were reduced (P＜0.05), and the compactibility of hippocampal nerve cells of rats was significantly improved. The p38MAPK/GADPH ratio of each dose groups of CBLE was significantly different from that of the model group (P＜0.05). The results showed that high dose of CBLE not only alleviated epilepsy symptoms, but also had a protective effect on epileptic rat neurons, which was achieved by inhibiting the expression of p38MAPK protein.