| 血红蛋白在海藻酸钠膜中的电化学和类酶活性研究 |
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| 引用本文： | 李业梅,刘传银.血红蛋白在海藻酸钠膜中的电化学和类酶活性研究[J].化学世界,2010,51(11). |
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| 作者姓名： | 李业梅 刘传银 |
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| 作者单位： | 1. 郧阳师范高等专科学校化学系,湖北,丹江口,442700
2. 郧阳师范高等专科学校化学系,湖北,丹江口,442700;武汉大学,化学系,湖北,武汉,430072 |
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| 基金项目： | 湖北省教育厅重点科研项目 |
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| 摘 要： | 用海藻酸钠(SA)将血红蛋白(Hb)固定到热裂解石墨电极表面,制备了Hb-SA膜修饰电极。包埋在SA膜中的Hb在磷酸盐缓冲溶液和乙醇混合溶液中与电极直接传递电子,得到一对对称的血红蛋白辅基血红素Fe(Ⅲ)/Fe(Ⅱ)电对的可逆氧化还原峰。其式电势随缓冲溶液pH值增加而负移,且呈线性关系,这说明血红蛋白的电子传递过程伴随有质子的转移。并考察了Hb-SA膜修饰电极在缓冲溶液和乙醇混合溶液中对氧气、双氧水、一氧化氮和六氯乙烷的电催化性质。
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| 关 键 词： | 血红蛋白 海藻酸钠 水/有机混合溶液 电化学 电催化 |
Electrochemical and Electrocatalysis Behaviors of Hemoglobin Entrapped in Sodium Alginate Hydrogel Films |
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| LI Ye-mei,LIU Chuan-yin.Electrochemical and Electrocatalysis Behaviors of Hemoglobin Entrapped in Sodium Alginate Hydrogel Films[J].Chemical World,2010,51(11). |
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| Authors: | LI Ye-mei LIU Chuan-yin |
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| Abstract: | Hemoglobin was immobilized on edge-plane pyrolytic graphite(EPG) electrode by sodium alginate hydrogel.Hemoglobin entrapped in the sodium alginate film undergoes fast direct electron-transfer reaction.The formal potential(E~o′) is linearly dependent on solution pH,indicating that the electron transfer is proton-coupled.The catalytic reduction of oxygen,hydrogen peroxide and nitric oxide and dehalogenation of hexachloroethane by hemoglobin entrapped in sodium alginate hydrogel were also explored. |
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| Keywords: | hemoglobin sodium alginate aqueous-organic solvents mixture electrochemistry electrocatalysis |
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