多穗柯三叶苷对脂多糖诱导小鼠炎症反应的保护作用

探讨多穗柯三叶苷对脂多糖（lipopolysaccharide，LPS）诱导的小鼠炎症反应的抗炎症作用。以3、30、300 mg/（kg·d）3 个剂量组的多穗柯三叶苷灌胃小鼠3 d后，腹腔注射0.1 mg/kg LPS，观察三叶苷预处理对LPS诱导的炎症因子表达的影响。酶联免疫反应测定小鼠血清中炎症因子肿瘤坏死因子（tumor necrosis factor-α，TNF-α）和白细胞介素-6（interleukin-6，IL-6）的含量，实时定量聚合酶链式反应法检测肝组织中TNF-α和IL-6的mRNA水平，免疫组织化学染色检测小鼠肝组织中TNF-α和IL-6蛋白水平。结果表明，3 mg/（kg·d）三叶苷剂量组可显著抑制LPS诱导的小鼠血清中TNF-α和IL-6的分泌，降低肝组织中TNF-α和IL-6的表达水平。多穗柯三叶苷对小鼠的炎症反应具有一定的保护作用。

Protective Effect of Trilobatin against Lipopolysaccharide-Induced Inflammatory Response in Mice

This study aimed to investigate the in vivo anti-inflammatory activity of trilobatin extracted from the young leaves
of Lithocarpus polystachyus Rehd on lipopolysaccharide (LPS)-induced inflammatory response in mice. After being treated
intragastrically with trilobatin at three different dosages (3, 30 and 300 mg/(kg·d)) for 3 days, the mice were administered
intraperitoneally with LPS (0.1 mg/kg) 1 h after the last intragastrical treatment on day 3. The effect of trilobatin on the
expression of LPS-induced pro-inflammatory cytokines was detected. Tumor necrosis factor-α (TNF-α) and interleukin-6
(IL-6) in serum were measured using enzyme-linked immunosorbent assay (ELISA) and the mRNA expression levels of
TNF-α and IL-6 in liver were assayed with real-time quantitative polymerase chain reaction (RT-qPCR). The protein levels
of TNF-α and IL-6 in liver were detected by immunohistochemical strept avidin-biotin complex (SABC) method. We found
that trilobatin at low dosage (3 mg/(kg·d)) significantly inhibited LPS-induced TNF-α and IL-6 production in serum, and
decreased their expression in liver. These results indicate that trilobatin from Lithocarpus polystachyus Rehd has protective
effect on inflammatory response in mice.