葡萄籽中活性成分提取工艺的研究

采用V (丙酮 )∶V (水 ) =2∶3和V(乙酸乙酯 )∶V(水 ) =8 5∶1 5两种溶剂体系从葡萄籽中提取活性成分。对比分析了两种体系的优劣 ,结果表明 :丙酮 -水体系提取过程复杂 ,提取率为0 2 0 %～ 0 35 % ,产物质量分数为 95 %～ 97% ,提取周期为 48h ;乙酸乙酯 -水体系选择性好 ,省去了除单宁步骤 ,使操作大为简化 ,提取率为 0 .30 %～ 0 .6 0 % ,产物质量分数为 97%～ 98% ,提取周期为 30h ,具有明显的优越性。以氯仿作沉淀剂 ,消除了叶绿素的干扰 ,提高了产物纯度。

Study of the Technology for Extracting Active Components From Grape Seeds

The active components (oligomeric flavanols,OF) in grape seeds were extracted with two solvent systems of V(acetone)∶ V (water)=2∶3 and V (ethyl acetate)∶ V (water)=8 5∶1 5 separately.The results showed that the extraction process by acetone/water system was complicated and tedious (48 h),and the yields was 0.20%～0.35% with 95%～97% purity,while the extraction process by ethyl acetate/water system was simple and rapid (30 h),obviating the procedure of tannin removal.Having higher yield (0.40%～0.60%) and purity(97%～98%),the use of ethyl acetate/water system was obviously advantageous.In the present method,the interference of chlorophyll was eliminated by using chloroform as a precipitant,so that extracts of higher purity could be obtained.