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| PHB产生菌发酵条件的研究 | |
| 引用本文： | 涂毅,王玮玥,张弘,魏凯,来晨刚,曹军卫. PHB产生菌发酵条件的研究[J]. 化学与生物工程, 2011, 0(9): 67-70. DOI: 10.3969/j.issn.1672-5425.2011.09.018 |
| 作者姓名： | 涂毅 王玮玥 张弘 魏凯 来晨刚 曹军卫 |
| 作者单位： | 武汉东湖学院生命科学与化学学院,湖北武汉,430212 |
| 摘 要： | 从神农架林区采集的土样中分离出了可以在细胞内积累聚-β-羟丁酸( Poly-β-hydroxybutyrate,PHB)的菌株,分析其产生PHB的能力,并对其中3个菌株的生长曲线和PHB产量进行了比较,其中SNJD-3-I和SNJD-3-Ⅱ的PHB积累量均在第6d达到最大,第7d明显下降,而SNJD-3-Ⅲ的PHB产量... |
| 关 键 词： | PHB产生菌 分离 正交实验 |
Research of Fermentation Conditions of the Strains Producing PHB |
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| TU Yi,WANG Wei-yue,ZHANG Hong,WEI Kai,LAI Chen-gang,CAO Jun-wei. Research of Fermentation Conditions of the Strains Producing PHB[J]. Chemistry & Bioengineering, 2011, 0(9): 67-70. DOI: 10.3969/j.issn.1672-5425.2011.09.018 | |
| Authors: | TU Yi WANG Wei-yue ZHANG Hong WEI Kai LAI Chen-gang CAO Jun-wei |
| Affiliation: | TU Yi,WANG Wei-yue,ZHANG Hong,WEI Kai,LAI Chen-gang,CAO Jun-wei(College of Life Sciences and Chemistry,Wuhan Donghu University,Wuhan 430212,China) |
| Abstract: | The strains accumulating PHB(poly-β-hydroxybutyrate) in cells were isolated from the soil samples collected at Shenlongjia.The PHB production capacities and growth curves of three of the strains were analyzed.As results shown,PHB accumulation of strain SNJD-3-Ⅰ and strain SNJD-3-Ⅱ had reached the maximum on the sixth day and declined significantly on the seventh day.However,PHB accumulation of strain SNJD-3-Ⅲ increased continuously after declining in the growth curve.The strain SNJD-3-Ⅰ was tested as gram-positive bacteria by Gram stain,bacilli,with spore,and preliminary identified as Bacillus.The fermentation conditions for SNJD-3-Ⅰ PHB were optimized by orthogonal experiment.The experiment results showed that the optimum fermentation conditions were as follows:basic fermentation medium,glucose as carbon source,peptone as nitrogen source,carbon-nitrogen ratio of 2∶1,pH value of 7.0,32 ℃ and six days of shaking cultivation.On above conditions,the production of PHB was 14.72 g·L-1. |
| Keywords: | strains producing PHB isolation orthogonal experiment |
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