| 应用荧光微球技术进行免疫检测研究 |
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| 引用本文： | 张燕玲,陈超,杨慧,薛小平.应用荧光微球技术进行免疫检测研究[J].化学与生物工程,2011,28(11):84-86. |
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| 作者姓名： | 张燕玲 陈超 杨慧 薛小平 |
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| 作者单位： | 1. 西北工业大学生命学院,陕西西安,710072
2. 国家微检测系统工程技术研究中心,陕西西安,710069 |
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| 基金项目： | 国家自然科学基金资助项目(31000067); 西北工业大学基础科研基金资助项目(JC200921) |
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| 摘 要： | 以聚苯乙烯羧基荧光微球为固相载体,将人免疫球蛋白G(IgG)和猪血红蛋白(Hb)分别与微球偶联,应用Luminex-100TM荧光微球检测系统检测抗体IgG和抗原Hb与荧光微球的偶联效率.结果表明,采用100μg·mL-1抗体或抗原浓度可以获得较高的偶联效率,且检测快速灵敏.应用荧光微球进行抗原抗体免疫检测可行.
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| 关 键 词： | 荧光微球 抗体 抗原 免疫检测 |
Study on Immunoassay by Fluorescent Microsphere-Based Technology |
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| ZHANG Yan-ling,CHEN Chao,YANG Hui,XUE Xiao-ping.Study on Immunoassay by Fluorescent Microsphere-Based Technology[J].Chemistry & Bioengineering,2011,28(11):84-86. |
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| Authors: | ZHANG Yan-ling CHEN Chao YANG Hui XUE Xiao-ping |
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| Affiliation: | ZHANG Yan-ling1,CHEN Chao2,YANG Hui1,XUE Xiao-ping1 |
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| Abstract: | Human immunoglobin G(IgG) and swine hemoglobin(Hb) were coupled to the carboxylated polystyrene fluorescent microsphere used as solid carrier,and Luminex-100TM system was used to detect the coupling efficiency between fluorescent microsphere and IgG or Hb.Results showed that,the maximal coupling efficiency could be achieved by using 100 μg·mL-1 antibody or antigen,and the detection was fast and sensitive.It is feasible to apply fluorescent microsphere technology to immunodetection. |
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| Keywords: | fluorescent microsphere antibody antigen immunodetection |
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