| 大孔树脂吸附分离紫苏茎总黄酮的工艺研究 |
| |
| 引用本文： | 吴元锋,孟耀庭,毛建卫,黄俊,刘士旺.大孔树脂吸附分离紫苏茎总黄酮的工艺研究[J].杭州应用工程技术学院学报,2012(3):232-236. |
| |
| 作者姓名： | 吴元锋 孟耀庭 毛建卫 黄俊 刘士旺 |
| |
| 作者单位： | [1]浙江科技学院生物与化学工程学院,杭州310023 [2]浙江省农产品化学与生物加工技术重点实验室,杭州310023 |
| |
| 基金项目： | 浙江省科技计划项目（2009C32060） |
| |
| 摘 要： | 考察了大孔树脂对紫苏茎提取液中总黄酮的吸附性能,优化了吸附工艺参数。首先对D-101、AB-8、DM130、ADS-7和ADS-17共5种大孔树脂的静态吸附量和解析率进行了实验,选择AB-8为最佳吸附树脂;静态吸附表明,3h内吸附即可达到平衡。还考察了上样速率、上样质量浓度、洗脱液乙醇质量分数和洗脱速率对分离的影响,结果表明优化的条件为：上样速率为1BV/h,上样质量浓度为0.15mg/mL,洗脱液乙醇质量分数为70%,洗脱流速为2BV/h。在此条件下,总黄酮洗脱率为93.56%,总黄酮纯度可提高4.5倍。
|
| 关 键 词： | 紫苏茎 总黄酮 大孔树脂 吸附 |
Study on separation of total flavonoids from stems of Pefilla frutescens by macroporous resins |
| |
| WU Yuan-feng,MENG Yao-ting,MAO Jian-wei,HUANG Jun,LIU Shi-wang.Study on separation of total flavonoids from stems of Pefilla frutescens by macroporous resins[J].Journal of Hangzhou Institute of Applied Engineering,2012(3):232-236. |
| |
| Authors: | WU Yuan-feng MENG Yao-ting MAO Jian-wei HUANG Jun LIU Shi-wang |
| |
| Affiliation: | 1. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China; 2. Zhejiang Provincial Key Laboratory or Chemical and Biological Processing Technology of Agricultural Products, Hangzhou 310023, China) |
| |
| Abstract: | The absorption property of pefilla frutescens total flavonoids on macroporous resins were studied, and the adsonrption parameters were optimized. Among five types of macroporous adsorption resin chosen, the results showed that AB-8 type maeroporous resin is suitable for separating Pefilla flavonoids. Static adsorption showed that adsorption equilibrium can be reached in 3 h. Then the effects of sampling concentration, sampling rate, eluting eoneentrantion and velocity on the separation were examined, the optimun conditions was： sampling rate 1 BV/h, sampling concentration 0. 15 mg/mL, eluting concentration 70~, eluting velocity 2 BV/h. Under this condition, the elution rate of flavone is 93.56%, the purity of flavone can be improved 4.5 times. |
| |
| Keywords: | stems of Pefilla frutescens total flavonoids macroporous resin adsorption |
| 本文献已被 维普 等数据库收录！ |
|
