植物发酵液对D-半乳糖致衰老模型小鼠的抗氧化活性研究

本文采用D-半乳糖皮下注射处理小鼠构建衰老动物模型,分别以不同性别小鼠血清和肝脏中三个特征性抗氧化酶SOD、CAT、GSH-Px活力及MDA含量作为评价指标,分析了植物发酵液(酵素)样品对衰老模型小鼠体内抗氧化活性的影响,结果表明,与模型组比较,灌胃剂量为1.5 m L/kg的酵素样品组能显著提高模型小鼠(雌性和雄性)中血清和肝脏中SOD、CAT、GSH-Px等抗氧化酶的活力,并降低MDA含量水平。同时,本文以脾脏指数、胸腺指数为指标初步评估酵素样品对小鼠体内免疫力的影响,结果显示灌胃剂量为0.24 m L/kg和0.6 m L/kg的酵素样品组都能适当提高小鼠的脾脏指数和胸腺指数。综合上述结果可知,本研究所述植物发酵液(酵素)样品具有良好的增强体内抗氧化活性作用,具有预防衰老的功效,并可能具备潜在的增强免疫力作用。

Antioxidant Activity of Fermented Plant Extracts (FPE) in a Mouse Model of D-Galactose-induced Aging

Mice were administered subcutaneous injection of D-galactose to produce an animal model of aging, which was used to study the in vivo effect of fermented plant extract (FPE) on antioxidant activity. Parameters including the activity of three characteristic antioxidant enzymes, namely superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) as well as the malondialdehyde (MDA) content in the serum and liver of mice of both sexes were extensively evaluated. Meanwhile, spleen index and thymus index were used as the indicators for the preliminary assessment of the impact of FPE on mice immunity in vivo. The results showed that compared with the model group, the activities of SOD, CAT, and GSH-Px in serum and liver of mice (both sexes) were significantly enhanced and corresponding MDA content was decreased in the group that received 1.5 mL/kg FPE gavage. Additionally, spleen and thymus indices of mice were increased to some extent in groups that received 0.24 and 0.6 mL/kg FPE gavage. Thus, FPE enhanced antioxidant activities in vivo, implying an anti-aging effect and may have potential to strengthen the immune system.