| HPLC法测定马来酸氯苯那敏中间体CPA的含量 |
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| 引用本文： | 王桂艳.HPLC法测定马来酸氯苯那敏中间体CPA的含量[J].辽宁化工,2009,38(6):420-421. |
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| 作者姓名： | 王桂艳 |
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| 作者单位： | 沈阳新地药业有限公司,辽宁,沈阳,110000 |
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| 摘 要： | 建立HPLC方法测定马来酸氯苯那敏中间体CPA（以下简称CPA）的含量。采用迪马Diamonsil（TM）C18（5μm×4．6mm×250mm）色谱柱；w（乙腈）：w（甲醇）：w（四氢呋喃）：w（水）=（27：8：5：60）（pH=3．5）为流动相，流速为1．2mL／min，检测波长为254nm，进样体积为20μL。结果显示：CPA的进样量在0—10．12μg范围内与主峰面积呈良好的线性关系，r=0．9999。平均回收率为99．4％，RSD为0．23％（n=9）。该方法简便、灵敏、准确度高，适用于CPA的含量测定。
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| 关 键 词： | 含量 CPA 高效液相色谱法 |
Quantitative Determination of CPA by HPLC |
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| WANG Gui-yan.Quantitative Determination of CPA by HPLC[J].Liaoning Chemical Industry,2009,38(6):420-421. |
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| Authors: | WANG Gui-yan |
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| Affiliation: | WANG Gui-yan |
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| Abstract: | HPLC methods for the determination of CPA was established. Diamonsil（TM）C18 （5 μm ×4.6 mm×200 mm） was used, and the mobile phase was composed of acetonitrile - methanol - tetrahydrofuran - water （ pH 3.5 ） （27： 8：5： 60）. The detection wavelength was at 254 nm with the flow rate of 1.2 mL/min. The results show the linear range is 0 - 10. 12 μg for CPA. r = 0.999 9. The average recoveries of CPA are 99.4% , RSD = 0.23% （n = 9）. This method is rapid, simple and accurate. |
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| Keywords: | CPA |
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