Construction of biosensors using a gold-binding polypeptide and a miniature integrated surface plasmon resonance sensor |
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Authors: | RG Woodbury C Wendin J Clendenning J Melendez J Elkind D Bartholomew S Brown CE Furlong |
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Affiliation: | Department of Medicine, University of Washington, Seattle 98195, USA. |
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Abstract: | Surface plasmon resonance (SPR) biosensors were constructed on miniature integrated sensors. Recognition elements were attached to the sensor surface using a gold-binding repeating polypeptide. Biosensors with fluorescyl groups attached to their surfaces were functional for at least 1 month of daily use with little decrease in response to the binding of an anti-fluorescyl monoclonal antibody. The coupling of protein A to the gold-binding polypeptide on the sensor surface enabled the biosensor to detect the binding of antibodies to the protein A and provided a sensor with convertible specificity. The system described herein provides a simple and rapid approach for the fabrication of highly specific, durable, portable and low cost SPR-based biosensors. |
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