Production of lactose-hydrolyzed milk using ethanol permeabilized yeast cells |
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| Authors: | Reeba Panesar Parmjit S. Panesar Ram S. Singh John F. Kennedy Manav B. Bera |
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| Affiliation: | 1. Department of Biotechnology, Punjabi University, Patiala 147 002, India;2. Department of Food Technology, Sant Longowal Institute of Engineering and Technology, Longowal 148 106, India;3. Birmingham Carbohydrate and Protein Technology Group, School of Chemistry, University of Birmingham, Birmingham B15 2TT, UK;4. Chembiotech Laboratories, Institute of Research and Development, University of Birmingham, Birmingham Research Park, Vincent Drive, Birmingham B15 2SQ, UK |
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| Abstract: | To overcome the problem of enzyme extraction and poor permeability of cell membrane to lactose, experimentation was carried to permeabilize Kluyveromyces marxianus NCIM 3465 cells for their subsequent use for the production of lactose-hydrolyzed milk. Different process parameters, such as biomass load, temperature, agitation and treatment time, were optimized for maximum lactose hydrolysis in skim milk using these cells. The ethanol-permeabilized yeast cells gave 89% hydrolysis of milk lactose under optimized conditions. |
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| Keywords: | Yeast Permeabilization Skim milk Lactose hydrolysis |
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