3D NIR‐II Molecular Imaging Distinguishes Targeted Organs with High‐Performance NIR‐II Bioconjugates |
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Authors: | Shoujun Zhu Sonia Herraiz Jingying Yue Mingxi Zhang Hao Wan Qinglai Yang Zhuoran Ma Yan Wang Jiahuan He Alexander L Antaris Yeteng Zhong Shuo Diao Yi Feng Ying Zhou Kuai Yu Guosong Hong Yongye Liang Aaron J Hsueh Hongjie Dai |
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Affiliation: | 1. Department of Chemistry, Stanford University, Stanford, CA, USA;2. Program of Reproductive and Stem Cell Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, Stanford, CA, USA;3. IVI Foundation, Valencia, Spain;4. Department of Materials Science & Engineering, South University of Science & Technology of China, Shenzhen, China;5. Research Center for Advanced Materials and Biotechnology, Research Institute of Tsinghua University in Shenzhen, Shenzhen, China |
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Abstract: | Greatly reduced scattering in the second near‐infrared (NIR‐II) region (1000–1700 nm) opens up many new exciting avenues of bioimaging research, yet NIR‐II fluorescence imaging is mostly implemented by using nontargeted fluorophores or wide‐field imaging setups, limiting the signal‐to‐background ratio and imaging penetration depth due to poor specific binding and out‐of‐focus signals. A newly developed high‐performance NIR‐II bioconjugate enables targeted imaging of a specific organ in the living body with high quality. Combined with a home‐built NIR‐II confocal set‐up, the enhanced imaging technique allows 900 µm‐deep 3D organ imaging without tissue clearing techniques. Bioconjugation of two hormones to nonoverlapping NIR‐II fluorophores facilitates two‐color imaging of different receptors, demonstrating unprecedented multicolor live molecular imaging across the NIR‐II window. This deep tissue imaging of specific receptors in live animals allows development of noninvasive molecular imaging of multifarious models of normal and neoplastic organs in vivo, beyond the traditional visible to NIR‐I range. The developed NIR‐II fluorescence microscopy will become a powerful imaging technique for deep tissue imaging without any physical sectioning or clearing treatment of the tissue. |
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Keywords: | 3D NIR‐II molecular imaging high‐performance NIR‐II bioconjugate live ovary imaging multicolor live molecular imaging NIR‐II confocal |
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