RP-HPLC法测定水果中的蛇麻醇酯

应用反相高效液相色谱法(RP-HPLC)建立蛇麻醇酯简便的分析方法。分析条件为：色谱柱Nucleosil C18(250mm×4.6mm，5μm)，流动相A为甲醇和水(甲醇:水=20:80)，用体积分数50%的磷酸调pH值至3.2±0.05，流动相B为甲醇(含0.01%的磷酸)，A:B=4:96，流速1.2mL/min，检测波长为210nm，柱温40℃。蛇麻醇酯的标准曲线为5～250μg/mL 7个治疗质量浓度梯度，y=4.6068x＋11.2640，R2=0.9992。精密度实验结果：连续5次进样，保留时间 RSD为0.2107%，峰面积的RSD为1.2843%；同一样品，在0～48h内9次进样，保留时间的RSD为0.3383%，峰面积的RSD为1.9737%。准确度实验结果：回收率为94.971%～101.964%，回收率的RSD为2.5825%。仪器的最低检出限为1.8886μg/mL。被测定的几种水果(青果、草莓、香蕉、巨峰葡萄、美国红提、番木瓜)中，以青果(干粉)中含量最高，达359μg/g以上。本研究建立的分析条件，能满足水果等样品中蛇麻醇酯定性定量分析的需要。

Determination of Lupeol in Fruits by RP-HPLC

A simple method was developed for determining lupeol in fruits by reverse-phase high performance liquid chromatography (RP-HPLC). Separation was carried out on reversed-phase Nucleosil C18 column (5μm, 250 mm × 4.6 mm i.d.) by using a mixture of methanol and water (20:80, pH 3.2 ± 0.05 adjusted by 50% phosphoric acid) as mobile phase A, and methanol containing 0.01% phosphoric acid as mobile phase B (A:B = 4:96) at a flow rate of 1.2 mL/min. The column temperature was hold at 40 ℃, and the detection wavelength was set as 210 nm. The correlation coefficient of the linear calibration curve (y = 4.6068x + 11.2640) was 0.9992 at the concentration range of 5 to 250μg/mL. The relative standard deviations (RSDs) of retention time and peak area in precision experiments for 5 replicate analyses and 9 replicate analyses in 48 h were 0.2107% and 1.2843%, and 0.3383% and 1.9737%, respectively. The recovery rate range was between 94.971% and 101.964% with a RSD of 2.5825%. The detection limit of this method was 1.8886μg/mL. The content of lupeol in dried Chinese olive powder was 359 μg/g, which was higher than that in strawberry, banana, Jufeng grape, American red grape and papaya. Therefore, the developed method is practicable for qualitative and quantitative determination of lupeol in fruits.