Reversible Modulation of Aptamer-Ligand Binding in RNA Light-Up Aptamers Containing G-Quadruplex Using Chemical Stimuli |
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Authors: | Rashi Soni A Murali Krishna Shahaji H More Dr Ashwani Sharma |
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Affiliation: | Department of Chemistry, Indian Institute of Science Education and Research (IISER) Tirupati, Tirupati, 517507 Andhra Pradesh, India |
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Abstract: | Regulating a system in equilibrium transiently to out-of-equilibrium by using certain stimuli is the strategy used by natural biomolecules to function. Herein, we showed that the interaction of synthetic RNA aptamers, having a G-quadruplex core structure, with their corresponding ligands could be regulated from their equilibrium state to non-equilibrium state in a reversible manner using simple chemical stimuli (Ag+ and cysteine). The approach would be useful for designing aptamer regulators that work in a dynamic nucleic acid network, where a strict control on aptamer-ligand interaction is needed. In addition, to the best of our knowledge, this is the first report which shows that RNA G-quadruplexes can be disrupted by the addition of silver ions. This would be useful not only in designing RNA-based sensors or regulators but would also be useful for understanding the role of metal ions in RNA folding and catalysis. |
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Keywords: | bio-thiols G-quadruplexes light-up aptamers RNA spinach aptamers |
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