大孔吸附树脂分离纯化猪毛菜总黄酮及其抗氧化活性

采用不同大孔吸附树脂分离纯化猪毛菜总黄酮,并对纯化后的总黄酮进行体外抗氧化活性测试。通过考察影响树脂静态和动态吸附与洗脱的主要因素,确定猪毛菜总黄酮分离纯化优化工艺条件。静态吸附实验表明,AB-8树脂分离纯化效果较好,并且吸附符合Langmuir和Freundlich方程。动态吸附和解吸的最佳工艺条件为:上样液质量浓度1.25 g/L、p H=4.5、上样流速2 m L/min、上样量2.5 BV(BV指树脂柱内装载树脂的体积)、洗脱剂为体积分数80%的乙醇溶液、洗脱流速1.0 m L/min,洗脱剂用量4 BV。所得洗脱液中黄酮质量分数从纯化前10.20%增加到纯化后51.89%,回收率为84.43%。体外实验表明,纯化后的黄酮可以清除羟自由基和超氧阴离子自由基,并有较好的还原力。纯化后的黄酮可以作为一种潜在的天然抗氧化剂。

Separation and Purification of Total Flavonoids from Salsola collina with Macroporous Resins and Evaluation of Antioxidant Activities

Separation and purification of total flavonoids from Salsola collina using macroporous resins and the antioxidant activities in vitro of purified flavonoids were studied. Critical factors that influence the static and dynamic adsorption and desorption of total flavonoids were selected and optimized. The static tests indicated that AB-8 resin was appropriate and its adsorption data were well fitted to the Langmuir and Freundlich isotherms. The optimal conditions for dynamic adsorption and desorption were sample concentration of 1.25mg/mL, pH 4.5, flow rate of 2mL/min, sample loading amount of 2.5BV, and complete desorption with 4BV of 80% ethanol at 1mL/min. After the optimized purification, the purity of flavonoids was improved from 10.2% to 51.89%, and the recovery yield was 84.43%. The antioxidant tests revealed that the purified ?avonoids could scavenge hydroxyl and oxygen radicals and possessed strong reducing power. The optimum conditions for purifying flavonoids are simple and feasible, which could provide experimental basis for the industrial production. The results have demonstrated that the purified ?avonoids from Salsola collina can be used as a potential source of natural antioxidant.