RP—HPLC法测定(7-甲氧基-3,4-二氢-1-萘基)乙腈的含量及其有关物质

目的:采用反相高效液相色谱法测定(7-甲氧基-3,4-二氢-1-萘基)乙腈含量及其有关物质。方法:采用Diamonsil~(TM)(钻石)C_(18)(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(68:32),检测波长232nm,流速0.8mL/min。结果:此色谱条件下(7-甲氧基-3,4-二氢-1-萘基)乙腈与有关物质能达到有效分离,在40.48～161.92 μ g/mL范围内线性关系良好,r=0.9998;仪器精密度RSD为0.94%;方法重复性RSD为1.3%;最低检测限4.9ng。结论:该方法简便、准确、可行,可用于(7-甲氧基-3,4-二氢-1-萘基)乙腈的质量控制。

RP-HPLC determination of content of(7-methoxy-3,4-dihydro-1-naphthyl)acetonitrile and its related substances

Objective:A reversed phase high performance liquid chromatography method for the determination of content of (7 - methoxy-3,4 - dihydro -1 - naphthyl) acetonitrile and its related substances.Methods:The analysis was performed on Diamonsil C_(18) reversed - phase analytical column(250mm×4.6mm,5μm);the mobile phase consisted of a mixture of methanol -water(68 :32);The ultraviolet detection wavelength was set at 232nm.The flow rate was 0.8 mL/min.Results:(7 - methoxy-3,4 - dihydro -1 - naphthyl) acetonitrile and its related substances could be seperated well.The linearity of the (7 - methoxy-3,4 - dihydro -1 - naphthyl) acetonitrile curve was well correlated (r=0.9998) within the range of 40.48-161.92μg/mL.The instrument precision RSD was 0.94%;The method precision RSD was 1.3%;The detection limit was 4.9ng.Conclusions:The method appeared to be simple,accurate and reproducible;It can be used to control the quality of (7 - methoxy-3,4 - dihydro -1 - naphthyl) acetonitrile.