| 微生物发酵生产鼠李糖的摇瓶实验研究 |
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| 引用本文： | 浦跃武,张浩嘉,梁世中.微生物发酵生产鼠李糖的摇瓶实验研究[J].河南工业大学学报(自然科学版),1999,20(2). |
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| 作者姓名： | 浦跃武 张浩嘉 梁世中 |
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| 作者单位： | 华南理工大学生物工程系，广州市食品工业研究所 |
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| 基金项目： | 广州市科委重点科研项目资助 |
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| 摘 要： | 将选育出的鼠李糖高产菌株，通过摇瓶培养确定了该菌株的最佳生长条件及产鼠李糖条件。培养基配方（ｇ／Ｌ）：ＮａＮＯ３７，ＮａＣｌ１．１，ＫＣｌ１．１，Ｋ２ＨＰＯ４４．４，ＭｇＳＯ４０．５，Ｃａ（ＮＯ３）２０．０１，ＫＨ２ＰＯ４３．４，ＦｅＳＯ４０．０００２，玉米油１４０ｍＬ，金属离子液５ｍＬ，ｐＨ值６５５；培养温度：３７℃；培养天数：７ｄ；摇床转速：２００ｒ／ｍｉｎ；接种量：２％。鼠李糖产量为４５～５５ｇ／Ｌ
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| 关 键 词： | 铜绿假单孢菌 鼠李糖 鼠李糖脂 |
STUDY ON MICROBIAL PRODUCING RHAMNOSE BY SHAKING FLASH CULTURE |
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| Pu YuewuZhang HaojiaLiang Shizhong Cao Chiping Wu Huixun Lai Suiwen.STUDY ON MICROBIAL PRODUCING RHAMNOSE BY SHAKING FLASH CULTURE[J].Journal of Henan University of Technology Natural Science Edition,1999,20(2). |
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| Authors: | Pu YuewuZhang HaojiaLiang Shizhong Cao Chiping Wu Huixun Lai Suiwen |
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| Affiliation: | Pu Yuewu1Zhang Haojia1Liang Shizhong1 Cao Chiping2 Wu Huixun2 Lai Suiwen2 |
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| Abstract: | In this article,the authors studied the shaking flask culture conditions of mutated Pseudomonas aeruginosa which have the high productive of rhamnose.The potimum culture conditions were defined,culture base(g/L) were composed of NaNO3 7,NaCl 1.1,KCl 1.1,K2HPO4 4.4,MgSO40.5,Ca(NO3)2 0.01,KH2PO4 3.4,FeSO4 0.0002,140mL corn oil,5mL trace metal solution,pH worth was 6.55,culture temperature was 37,fermentation time was days,rotary shaking rate was 200r/min,inoculum size was 2%.The rhamnose productive of the mutant was 4.55.5g/L |
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| Keywords: | Pseudomonas aeruginosa rhamnose rhamnolipid |
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