温度和浊度对罗丹明B示踪检测的影响及校正

由于环境污染日益严重，对污染物进行追踪具有必要性，使用示踪剂罗丹明B进行追踪实验是对污染物溯源的一种有效方式，但在使用罗丹明B荧光传感器进行原位污染物追踪检测中，温度浊度等环境因素变化会对测量结果造成影响，因此需对温度和浊度两个主要环境因素进行补偿校正，从而提高罗丹明B检测准确度。利用荧光分光光度计检测不同浓度罗丹明B荧光光谱，并采用偏最小二乘法（PLS）进行光谱数据分析与建立标准曲线。测量并分析了温度10~60 ℃和浊度0~55 NTU范围的罗丹明B荧光光谱，结果表明罗丹明B的荧光强度与温度呈负相关性，与浊度呈正相关性。由于罗丹明B浓度差值变化率与温度和浊度呈良好线性关系，因此利用罗丹明B浓度差值变化率对不同环境进行补偿校正。在温度和浊度补偿校正之后，浓度检测结果的相对误差分别小于0.48%和0.34%，提高了罗丹明B在不同环境中的检测准确度，并对温度和浊度共同影响时的检测结果进行分析，建立共同补偿校正模型。为抑制温度和浊度对罗丹明B原位检测的干扰提供了一种修正思路。

Influence of temperature and turbidity on Rhodamine B tracer detection and correction

Due to the increasingly serious environmental pollution, it is necessary to trace the pollutants. The tracer Rhodamine B is an effective way to trace the source of pollutants. However, in the in-situ pollutant tracking and detection using Rhodamine B fluorescence sensor, the measurement results will be affected by environmental factors such as temperature and turbidity. Therefore, it is important that the accuracy of the in-situ detection of Rhodamine B is improved by compensating and correcting the two main environmental factors, temperature and turbidity. Fluorescence spectra of Rhodamine B with different concentrations were detected by fluorescence spectrophotometer, and partial least squares (PLS) method was used to analyze the spectral data and establish the standard curve. The fluorescence spectra of Rhodamine B were measured and analyzed in the range of temperature from 10 ℃ to 60 ℃ and turbidity from 0 NTU to 55 NTU. The results showed that the fluorescence intensity of Rhodamine B was negatively correlated with temperature and positively correlated with turbidity. Since the rate of change of Rhodamine B concentration difference has a good linear relationship with temperature and turbidity, the rate of change of Rhodamine B concentration difference is used for compensation correction in different environments. After temperature and turbidity compensation correction, the relative errors of concentration detection results were less than 0.48% and 0.34%, respectively, which improved the detection accuracy of Rhodamine B in different environments. What’s more, under the influence of temperature and turbidity, the detection results were analyzed, by doing so, a model of common compensation correction was established. It provides a correction method to suppress the interference of temperature and turbidity in-situ detection of Rhodamine B.