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REFLEXⅢ基质辅助激光解析电离时间飞行(MALDI-TOF)质谱仪的特点及应用
引用本文:王洪奇 黄舜彦. REFLEXⅢ基质辅助激光解析电离时间飞行(MALDI-TOF)质谱仪的特点及应用[J]. 现代仪器, 1999, 0(3): 3-6
作者姓名:王洪奇 黄舜彦
作者单位:布鲁克仪器有限公司,布鲁克仪器有限公司 北京 100081,北京 100081
摘    要:
REFLEAⅢ基质辅助激光解析电离时间飞行(MALDI-TOF)质谱仪(图1)是布鲁克公司推出的世界上最灵敏的MALDI-TOF质谱仪。该仪器配有自动制样机,无网离子的延迟引出技术,无网双聚焦反射器,除可测各种分子的分子量外,还可通过源后分解谱(Post Source decomposition,PSD)得到结构信息,是当前研究蛋白质组必不可少的质谱仪。本文对该仪器的主要优点,结构特点,仪器主要配置及应用等作了简要介绍。

关 键 词:基质辅助激光解析电离技术  飞行时间质谱仪  蛋白质组  蛋白质  多肽聚糖  核甘酸

The Characteristics and Applications of REFELEX I MALDI-TOF
Wang Hongqi Huang Shunyan. The Characteristics and Applications of REFELEX I MALDI-TOF[J]. Modern Instruments, 1999, 0(3): 3-6
Authors:Wang Hongqi Huang Shunyan
Abstract:
REFELEX Ⅲ MALDI-TOF is the most sensitive MALDI-TOF mass spectrometer (MS) in the world . With our patented gridless pulse ion extraction (PIE), and gridless double focusing reflec-tron design, it can measure molecular weight of a molecule with high precision and give structural information with post source decomposition (PSD) analysis. It is an ideal MS instrument for researchers especially those who are working on the proteome project. In this article , we will decribe some of the characteristics of the intrument , its basic design and some applications.
Keywords:MALDI-TOF MS proteome protein peptide glycans and oligonucleotides Automation
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