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Retention of β-galactosidase activity in crude cellular extracts from Lactobacillus delbrueckii ssp. bulgaricus 11842 upon drying
Authors:T Vasiljevic  P Jelen
Affiliation:Department of Agricultural, Food and Nutritional Science, 206 Agricultural/Forestry Center, University of Alberta, Edmonton, Alberta, Canada T6G 2P5
Abstract:Crude cellular extracts (CCEs) containing active β-galactosidase from Lactobacillus delbrueckii ssp. bulgaricus 11842 were spray-dried at three different outlet air temperatures (45, 55 or 65°C) or freeze-dried, with or without whey proteins, casein, whey or skim milk as drying adjuncts. The use of whey or skim milk resulted in significantly ( P   <  0.05) higher β-galactosidase activity retention in comparison to all other CCEs. This effect was not related to the initial total solids (TS) content (4–10%) of the feedstock solutions, but was presumably caused by the presence of lactose in the whey or skim milk CCE preparations.
Keywords:Activity retention  β-Galactosidase  Crude cellular extract  Freeze-drying                Lactobacillus bulgaricus              Spray-drying
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