| 重组猕猴桃果胶甲酯酶抑制剂的抑制活性研究 |
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| 引用本文： | 梅晓宏,高红岩,罗云波.重组猕猴桃果胶甲酯酶抑制剂的抑制活性研究[J].食品科技,2007,32(4):134-137. |
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| 作者姓名： | 梅晓宏 高红岩 罗云波 |
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| 作者单位： | 中国农业大学食品科学与营养工程学院,北京,100083 |
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| 摘 要： | 猕猴桃的果胶甲酯酶抑制剂可有效抑制不同种类植物的果胶甲酯酶活性。主要研究在巴斯德毕赤酵母中成功表达的重组猕猴桃果胶甲酯酶抑制剂(被命名为kWPMEI)的抑制活性。通过研究发现,kWPMEI对桔子果胶甲酯酶的抑制活性最强,其次是胡萝卜,最弱的是番茄。同时kWPMEI对上述三种植物果胶甲酯酶的抑制活性受环境温度和pH的影响比较显著。
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| 关 键 词： | 果胶甲酯酶抑制剂 果胶甲酯酶 抑制活性 |
| 文章编号： | 1005-9989(2007)04-0134-04 |
| 修稿时间： | 2006年10月20 |
Study of inhibitory capacity of recombinant kiwi pectin methylesterase inhibitor |
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| MEI Xiao-hong,GAO Hong-yan,LUO Yun-bo.Study of inhibitory capacity of recombinant kiwi pectin methylesterase inhibitor[J].Food Science and Technology,2007,32(4):134-137. |
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| Authors: | MEI Xiao-hong GAO Hong-yan LUO Yun-bo |
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| Abstract: | The kiwi pectin methylesterase inhibitor can effectively inhibit pectin methylesterase of various plant sources. In this article, inhibitory capacity of the recombinant kiwi pectin methylesterase inhibitor (designated as kWPMEI), which had been successfully expressed in Pichia pastoris, was mainly studied. kWPMEI showed the strongest inhibitory capacity to pectin methylesterase of orange, and then carrot. kWPMEI showed the weakest inhibitory capacity to pectin methylesterase of tomato. At the same time, the inhibitory capacity of kWPMEI to pectin methylesterases of the above plants was greatly affected by pH and temperature. |
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| Keywords: | pectin methylesterase inhibitor pectin methylesterase inhibitory activity |
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