| A+C群脑膜炎球菌多糖结合疫苗的研制 |
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| 引用本文： | 黄镇,钱雯,袁琳,施競,陈燕,陈南萍,周红军,李文春,杨美峰,刘红岩.A+C群脑膜炎球菌多糖结合疫苗的研制[J].粉末涂料与涂装,2005,18(6):483-486. |
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| 作者姓名： | 黄镇 钱雯 袁琳 施競 陈燕 陈南萍 周红军 李文春 杨美峰 刘红岩 |
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| 作者单位： | [1]云南沃森生物技术有限公司,昆明650118 [2]云南大学药学院,昆明650091 [3]云南大学生物制药创新人才培养基地,昆明650091 |
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| 摘 要： | 目的研制A+C群脑膜炎球菌多糖-破伤风类毒素结合疫苗。方法A群和C群脑膜炎球菌多糖在溴化氰(CNBr)的活化下,以1,6己二酰肼(ADH)作为连接子,与精制破伤风类毒素(TT)在碳二亚胺(EDAC)作用下结合,再按一定的比例将两者混合,制成A+C群脑膜炎球菌多糖结合疫苗原液,加保护剂冻干后,制成A+C群脑膜炎球菌多糖结合疫苗。结果经检定,A+C群脑膜炎球菌多糖结合疫苗各项指标均达到了质控标准。该疫苗安全性及免疫原性良好。结论已成功研制出冻干A+C群脑膜炎球菌多糖结合疫苗,该制备工艺是可行的。
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| 关 键 词： | 脑膜炎球菌 结合疫苗 免疫原性 |
| 收稿时间： | 2004-11-08 |
| 修稿时间： | 2004年11月8日 |
Preparation of Group A + C Meningococcal Polysaccharide Conjugate Vaccine |
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| HUANG Zhen ,QIAN Wen,YUAN Lin,et al.Preparation of Group A + C Meningococcal Polysaccharide Conjugate Vaccine[J].Chinese Journal of Biologicals,2005,18(6):483-486. |
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| Authors: | HUANG Zhen QIAN Wen YUAN Lin |
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| Affiliation: | Wosen Biotechnology Co. Ltd, Kunming 650118, China |
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| Abstract: | Objective To prepare group A C meningococcal polysaccharide conjugate vaccine.MethodsActivate groups A and C meningococcal polysaccharide with cyanogens bromide(CNBr) and prepare into MenAPS-ADH and MenCPS-ADH derivatives by reacting with 1,6-adipic acid dihydrazine(ADH).Mix MenAPS-ADH and MenCPS-ADH derivatives with purified tetanus toxoid(TT) at a certain mass ratio,then add 1-ethyl-3(3-dimethylaminopropyl)-carbodiimide(EDAC) to prepare bulks of MenAPS-TT and MenCPSTT conjugates respectively.Mix the two bulks at a certain ratio,add stabilizer and lyophilize to prepare group A C meningococcal polysaccharide conjugate vaccine.Results(All the) indexes of the prepared A C meningococcal polysaccharide conjugate vaccine met the standard for quality control.The vaccine showed good safety and immunogenicity.Conclusion Freeze-dried A C meningococcal polysaccharide conjugate vaccine was successfully prepared,and the production procedure was feasible. |
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| Keywords: | Meningoccus Conjugate vaccine Immunogenicity |
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