反胶束萃取精氨酸脱亚胺酶

报道了用反胶束体系萃取精氨酸脱亚胺酶(ADI)的研究结果,为精氨酸脱亚胺酶的分离纯化提供了一种方法。在反胶束体系中采用十六烷基三甲基溴化铵(CTAB)作为表面活性剂,正辛烷和丁醇作为溶剂及助溶剂。考察了水相pH、振荡时间、离子强度、表面活性剂浓度及精氨酸脱亚胺酶质量浓度等因素对精氨酸脱亚胺酶分离纯化效果的影响。实验结果表明,当c(CTAB)=0.01 mol/L,pH=7,振荡时间15 min,体系中用于反萃取的c(NaCl)=0.75 mol/L,且起始粗酶质量浓度控制在30 g/L时,通过辛烷/丁醇/CTAB反胶束体系的萃取和反萃取,ADI酶液萃取率E达到85%,比活达到1.107 U/mg,是起始酶液的4.52倍。

Reversed Micellar Extraction of Arginine Deiminase

The result of extraction of arginine deiminase(ADI) using reversed micellar system is reported,which provides a method for the purification of arginine deiminase.In the system,cetyltrimethylammonium bromide(CTAB) was used as surfactant,octane was used as solvent and butanol was used as co-solvent.The optimum conditions were established that the concentration of CTAB is controlled at 0.01 mol/L,pH at 7,shaking time at 15 minutes,the concentration of NaCl at 0.75 mol/L,and the initial enzyme mass concentration at 30 g/L.The yield of ADI can reach 85%,while the specific activity can reach 1.107 U/mg which is 4.52 times that of the original deiminase.