生物转化中新酶应用研究的最新进展

目前,在生物转化中出现了越来越多与传统工艺大不相同的新工艺,特别是生物酶在生物转化中的应用。硫酸酯酶可以立体选择性和对映体选择性地催化水解仲烷基硫酸酯,并通过改变其构象从而产生纯手性的化合物;卤代醇脱卤酶可以催化多种非天然亲核性底物;在水介质中,裂解酶可以催化偶姻或苯偶姻反应从而产生非对称的碳-碳键;最近还出现了用酶催化生产纯手性α-L-氨基酸的新方法。

Recent Advances in New Enzymes Used in Biotransformations

Several novel bioprocesses that have little or no counterpart in traditional methodology have recently been reported,especially when enzymes was used in biotransformation. The stereoselective and enantioselective hydrolysis of sec-alkyl sulfate esters by alkyl sulfatases proceeds with inversion of configuration and furnishes a homochiral product mixture. Haloalcohol deh~logenases were shown to accept various non-natural nucleophiles. Asymmetric carbon,carbon bond formation via the acyloin- and benzoin-reaction was uccessfully catalyzed in water by novel lyases. New methods for the production of chiral nonracemic a-L-amino acids and amines were recently reported.