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| 乳品中致病大肠杆菌荧光双重PCR技术建立 | |
| 引用本文： | 郭清泉,廖如燕,曾旸.乳品中致病大肠杆菌荧光双重PCR技术建立[J].食品研究与开发,2012,33(2):131-133. |
| 作者姓名： | 郭清泉 廖如燕 曾旸 |
| 作者单位： | 1. 广东工业大学轻工化工学院,广东广州,510006 2. 广州市出入境检验检疫局,广东广州,510623 3. 汕头大学医学院,广东汕头,515041 |
| 基金项目： | 广东省科技计划项目(2008B021400006) |
| 摘 要： | 建立乳及乳制品中致病性大肠杆菌(EPEC)的快检方法。以EPEC特异的微绒毛粘连基因(eae gene)和茵毛束形成编码基因(bfp gene)为模板,设计两对引物,荧光探针对EPEC进行特异性扩增。该方法快速,特异性好,对EPEC的最低检出量为1 cfu/μL(纯菌),检出时间为3 h,同一样品重复检测3次ct值的变异系数均小于5%。建立可快速特异检测乳及乳制品中EPEC的双重荧光PCR方法。 |
| 关 键 词： | 两重荧光PCR 乳及乳制品 致病性大肠杆菌 |
Rapid Detection of Enteropathogenic Escherichia Coli by Double Fluorescence PCR Assay in Milk and Milk Product |
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| GUO Qing-quan , LIAO Ru-yan , ZENG Yang.Rapid Detection of Enteropathogenic Escherichia Coli by Double Fluorescence PCR Assay in Milk and Milk Product[J].Food Research and Developent,2012,33(2):131-133. | |
| Authors: | GUO Qing-quan LIAO Ru-yan ZENG Yang |
| Affiliation: | Yang3(1.Faculty of Chemical Engineering and Light industry,Guangdong University of Technology,Guangzhou 510006,Guangdong,China;2.Guangzhou ntry-exit Inspection and Quarantine Bureau,Guangzhou 510623,Guangdong,China;3.Medical College,Shantou University,Shantou 515041,Guangdong,China) |
| Abstract: | Establish a rapid method for detecting Enteropathogenic Escherichia coli(EPEC) in milk and milk product.Based on attaching and effacing lesion(eaeA gene) and bundle-forming pili(bfpA gene) of EPEC,two pairs of primers and Taqman-based fluorescent probe were designed.Then EPEC were detected by double fluorescent PCR assay.The method was rapid and specific.The limit of detection was 1 cfu/μL in 3 hours.Stability test showed that coefficient variables were all less than 5 %.A feasible,rapid,sensitive and specific double fluorescence PCR method was established to detect EPEC in milk an d milk product. |
| Keywords: | double fluorescence PCR milk and milk product Enteropathogenic Escherichia coli |
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