HPLC法同时测定食品接触橡胶密封垫圈中3种醛类化合物迁移量

目的:建立一种利用HPLC同时测定食品接触橡胶密封垫圈中甲醛、乙醛和丙烯醛迁移量的分析方法。方法:以4%乙酸水溶液、10%乙醇水溶液、20%乙醇水溶液、50%乙醇水溶液及橄榄油作为食品模拟物,按GB 5009.156—2016进行迁移试验,经衍生化反应后,采用Accucore RP-MS色谱柱分离,以乙腈—水溶液为流动相等度洗脱,紫外检测器检测。结果:3种醛类化合物在0.05~5.00 mg/L内呈良好的线性关系,相关系数R²≥0.9983。甲醛、乙醛和丙烯醛检出限分别为0.58,0.62,1.68 mg/kg;定量限分别为0.98,0.99,2.93 mg/kg,在1.0~15.0 mg/kg添加水平范围内,3种醛类化合物回收率为81.0%~103.3%;相对标准偏差为0.78%~7.05%。结论:该方法适用于食品接触橡胶密封垫圈中醛类化合物迁移量的检测。

Simultaneous determination on migration of three aldehydes in food contact rubber gaskets by HPLC

Objective:A HPLC method was established for simultaneous determination of formaldehyde,acetaldehyde and acrolein in food contact rubber gaskets.Methods:The migration test was carried out according to GB 5009.156—2016 by using 4% acetic acid aqueous solution,10% ethanol aqueous solution,20% ethanol aqueous solution,50% ethanol aqueous solution and olive oil as food stimulants.After derivatization reaction,the samples were separated on Accucore RP-MS column with acetonitrile-water solution as mobile phase and detected by UV detector.Results:The results showed that the three aldehydes showed good linear relationship in the mass concentration range of 0.05~5.0 mg/L,with the correlation coefficients R≥0.9983.The detection limits of formaldehyde,acetaldehyde and acrolein were 0.57,0.62 and 1.68 mg/kg,respectively.The limits of quantification were 0.96,0.99 and 2.93 mg/kg,respectively.In the range of 1.0~15.0 mg/kg,the recoveries of the three aldehydes were 81.0%~103.3%,respectively.The relative standard deviations were 0.78%~7.05% respectively.Conclusion:This method is suitable for the detection of the migration of aldehydes in food contact rubber gaskets.