| 高压微射流处理对大豆分离蛋白构象及功能特性的影响 |
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| 引用本文： | 沈兰,王昌盛,唐传核.高压微射流处理对大豆分离蛋白构象及功能特性的影响[J].食品科学,2012,33(3):72-76. |
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| 作者姓名： | 沈兰 王昌盛 唐传核 |
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| 作者单位： | 华南理工大学轻工与食品学院 |
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| 基金项目： | 国家自然科学基金项目(30972049) |
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| 摘 要： | 通过测定溶解度、乳化性、疏水性和运用差示扫描量热仪分析(DSC)和圆二色光谱(CD)分析探讨不同压力微射流处理(HPM)对大豆分离蛋白(SPI)构象和功能特性的影响。HPM诱导不溶性蛋白聚合物解聚，增加SPI的溶解度、乳化活性指数和疏水性。DSC图谱结果表明，处理后的SPI变性温度基本维持不变，但变性焓值呈现下降趋势。CD光谱分析表明，HPM对SPI的二级、三级结构没有明显的影响。
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| 关 键 词： | 大豆分离蛋白 高压微射流 功能特性 构象 |
Effect of High Pressure Microfluidization on Functional and Conformational Properties of SPI |
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| SHEN Lan,WANG Chang-sheng,TANG Chuan-he.Effect of High Pressure Microfluidization on Functional and Conformational Properties of SPI[J].Food Science,2012,33(3):72-76. |
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| Authors: | SHEN Lan WANG Chang-sheng TANG Chuan-he |
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| Affiliation: | College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China |
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| Abstract: | The effect of high pressure microfluidization(HPM) on functional properties and conformational properties of soy protein isolated(SPI) was investigated by measuring solubility,emulsification,hydrophobic properties,DSC thermogram and UV-CD spectrum.HPM could cause insoluble aggregates to dissociate,thus improving the solubility,emulsifying activity index(EAI) and hydrophobic properties of SPI.DSC analysis indicated Td remained basically unchanged,but ΔH tended to decline.UV-CD spectrum analysis showed that both the tertiary conformation and secondary structure of SPI were nearly unaffected by HPM treatment. |
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| Keywords: | soy protein isolated(SPI) high pressure microfluidization functional properties conformation |
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