| 生物自显影技术在荸荠英抑菌成分分离中的应用 |
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| 引用本文： | 郝淑贤,黄卉,刘欣,陈永泉.生物自显影技术在荸荠英抑菌成分分离中的应用[J].食品与发酵工业,2004,30(5):88-91. |
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| 作者姓名： | 郝淑贤 黄卉 刘欣 陈永泉 |
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| 作者单位： | 1. 南海水产研究所营养与食品工程研究室,广州,510300
2. 华南农业大学,广州,510642 |
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| 摘 要： | 应用硅胶板层析技术,以枯草芽孢杆菌作为指示菌,研究生物自显影对荸荠英提取物抑菌成分的活性跟踪方法。用200 mg/mL荸荠英提取物丙酮溶液点样4μL,以二元溶剂V(CCl_4):V(甲醇)=8.5:1.5展开,贴板10min,37℃培养10h即可得到清晰的抑菌斑色谱图。应用该方法可将荸荠英粗提物乙酸乙酯部分分离得到6个活性组分。
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| 关 键 词： | 荸荠英 生物自显影 分离 分离度 |
| 修稿时间： | 2003年12月22 |
Application of Biological Development Technique in Qualitative Analysis for Anrimicrobial Components of Puchiin Extract |
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| Hao Shuxian,Huang Hui,Liu Xin,Chen Yongquan Nutrition and Food Engineering Division,South China Sea Fisheries Research Institute,Guangzhou, South China Agriculture University,Guangzhou.Application of Biological Development Technique in Qualitative Analysis for Anrimicrobial Components of Puchiin Extract[J].Food and Fermentation Industries,2004,30(5):88-91. |
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| Authors: | Hao Shuxian Huang Hui Liu Xin Chen Yongquan Nutrition and Food Engineering Division South China Sea Fisheries Research Institute Guangzhou South China Agriculture University Guangzhou |
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| Affiliation: | Hao Shuxian,Huang Hui,Liu Xin,Chen Yongquan Nutrition and Food Engineering Division,South China Sea Fisheries Research Institute,Guangzhou,510300 South China Agriculture University,Guangzhou,510642 |
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| Abstract: | The biological development technique was used to provide a qualitative analysis method
for tracking anti-microbial components in puchiin. In the study, the Bacillus subtilis was regarded as
indicator organism. The optimum condition was obtained with 200mg/mL extract in acetone solution:
sample volume of 4μL, diffusion time of 10min and culture time of 10h at 37℃. Six different antibac-
terial points appeared using the solution of chloroform and carbinol with the ratio of 8.5 to 1.5. |
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| Keywords: | puchiin biological development isolation R_f |
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