大叶藻总黄酮的大孔树脂纯化工艺

为纯化大叶藻中提取的总黄酮，选择5 种大孔吸附树脂，通过静态吸附和解吸实验，选定两种最优树脂D101-1和AB-8；再将两种树脂进行混合实验，选出混合吸附树脂最优混合比例，最后确定最佳纯化工艺条件：D101-1和AB-8吸附树脂按2∶3比例混合、上样液pH 3、样液质量浓度1.25 mg/mL、洗脱液乙醇体积分数70%，上样量和上样流速分别为6 BV和3 BV/h，洗脱体积和洗脱流速分别为5 BV和3 BV/h条件下进行纯化实验，样液中的总黄酮含量由原来（12.66±0.42）%上升至（51.25±1.26）%。

Purification of Flavonoids from Zostera marina L. by Macroporous Resin Adsorption

Macroporous resins D101-1and AB-8 were found more suitable to purify flavonoids from Zostera marina L., as
indicated by comparing their dynamic adsorption and desorption capacities with those of three other kinds of macroporous
resin. The purification process was optimized by combined use of the two selected resins. The optimal mixing ratio of D101-1
to AB-8 was 2:3. The best purification results were achieved by loading 6 BV (bed volume) of the sample containing 1.25 mg/mL
flavonoids at pH onto the mixed column at a flow rate of 3 BV/h and subsequently eluting the column with 5 BV of 70%
ethanol at a flow rate of 3 BV/h. After the purification, the total flavonoid content was increased from (12.66 ± 0.42)% to
(51.25 ± 1.26)%.