| 大孔树脂法纯化锦灯笼宿萼总黄酮的工艺 |
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| 引用本文： | 王晓林,薛健飞,陈帅,王慧竹,钟方丽.大孔树脂法纯化锦灯笼宿萼总黄酮的工艺[J].食品科学,2014,35(14):58-61. |
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| 作者姓名： | 王晓林 薛健飞 陈帅 王慧竹 钟方丽 |
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| 作者单位： | 吉林化工学院化学与制药工程学院，吉林 吉林 132022 |
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| 基金项目： | 吉林省教育厅资助项目(吉教科合字[2014]第355号) |
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| 摘 要： | 以总黄酮吸附率、解吸率为指标，在单因素试验基础上，利用五因素四水平正交试验研究D-101型大孔吸
附树脂纯化锦灯笼宿萼总黄酮的工艺条件。结果表明：pH 10的锦灯笼宿萼总黄酮提取液、树脂吸附3 h、并用体积
35 倍于树脂质量（解吸液体积/树脂质量）体积分数80%的乙醇溶液解吸7 h时，锦灯笼宿萼浸膏中总黄酮的含量由
11.45%提高到45.56%。
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| 关 键 词： | 锦灯笼 总黄酮 大孔吸附树脂 纯化 |
Purification of Total Flavonoids from Persistent Calyx of Physalis alkekengi L.var. franchetii (Mast.) Makino Using Macroporous Resin |
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| WANG Xiao-lin,XUE Jian-fei,CHEN Shuai,WANG Hui-zhu,ZHONG Fang-li.Purification of Total Flavonoids from Persistent Calyx of Physalis alkekengi L.var. franchetii (Mast.) Makino Using Macroporous Resin[J].Food Science,2014,35(14):58-61. |
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| Authors: | WANG Xiao-lin XUE Jian-fei CHEN Shuai WANG Hui-zhu ZHONG Fang-li |
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| Affiliation: | School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China |
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| Abstract: | The purification of total flavonoids extracted from the persistent calyx of Physalis alkekengi L. var. franchetii
(Mast.) Makino using D-101 macroporous resin was optimized by an orthogonal array design involving five independent
variables at four different levels using adsorption and desorption rates of total flavonoids as response variables. The purity of
total flavonoids was increased from 11.45% to 45.56% when the total flavonoid extract from the persistent calyx of Physalis
alkekengi L. var. franchetii (Mast.) Makino at pH 10 was adsorbed by the resin for 3 h and then desorbed with a 35-fold
volume of 80% ethanol for 7 h. |
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| Keywords: | Physalis alkekengi L var franchetii (Mast ) Makino total flavonoid macroporous resin purification |
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