| 软枣猕猴桃根中蒽醌类化合物的分离纯化和含量测定 |
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| 引用本文： | 杨玉红,李鑫,杨建,刘长江,康宗利.软枣猕猴桃根中蒽醌类化合物的分离纯化和含量测定[J].食品科学,2014,35(16):124-127. |
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| 作者姓名： | 杨玉红 李鑫 杨建 刘长江 康宗利 |
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| 作者单位： | 1.沈阳工学院生命工程学院，辽宁 抚顺 113122；2.沈阳农业大学生物科学技术学院，辽宁 沈阳 110866；
3.沈阳农业大学食品学院，辽宁 沈阳 110866 |
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| 基金项目： | 公益性行业(农业)科研专项(200903013) |
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| 摘 要： | 利用聚酰胺柱层析和硅胶柱层析法从软枣猕猴桃根中分离纯化蒽醌化合物，采用高效液相色谱法分析法检测软枣猕猴桃根中蒽醌类化合物的含量。结果表明：分离的最佳条件为200 目硅胶、φ1.1 cm×100 cm层析柱、洗脱剂流速1.5 mL/min；甲醇-水（90∶10，V/V）流动相、254 nm检测波长的高效液相色谱测定软枣猕猴桃根中蒽醌主要组分为大黄素和大黄酚，其含量分别为46 μg/g和157.5 μg/g。
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| 关 键 词： | 软枣猕猴桃根 蒽醌 分离纯化 检测 |
Isolation,Purification and Detection of Anthraquinone Compounds from the Root of Actinidia arguta Sieb. et Zucc. |
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| YANG Yu-hong,LI Xin,YANG Jian,LIU Chang-jiang,KANG Zong-li.Isolation,Purification and Detection of Anthraquinone Compounds from the Root of Actinidia arguta Sieb. et Zucc.[J].Food Science,2014,35(16):124-127. |
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| Authors: | YANG Yu-hong LI Xin YANG Jian LIU Chang-jiang KANG Zong-li |
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| Affiliation: | 1. College of Life Engineering, Shenyang Institute of Technology, Fushun 113122, China;
2. College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China;
3. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China |
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| Abstract: | Anthraquinones from the root of Actinidia arguta Sieb. et Zucc. were isolated and purified by polyamide column
chromatography and silica gel column chromatography and were determined by high performance liquid chromatography
(HPLC). The results showed that the optimum silica gel column chromatographic conditions were selected as 200 mesh silica
gel, column dimensions φ 1.1 cm × 100 cm, flow rate 1.5 mL/min and mobile phase composed of a mixture of methanol
and water (90:10, V/V). The detection wavelength for anthraquinones was 254 nm. Emodin and chrysophanol as the major
anthraquinones were detected in the root of Actinidia arguta Sieb. et Zucc. at levels of 46 and 157.5 μg/g, respectively. |
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| Keywords: | root of Actinidia arguta Sieb et Zucc anthraquinone isolation and purification detection |
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