异丙酚 、氯胺酮对大鼠突触体 Na+, K+-ATP 酶活性的影响*

目的 观察异丙酚、氯胺酮对大鼠不同脑区突触体 Na⁺, K⁺-AT P 酶活性的影响。方法 SD 大鼠, 随机分为三组, 每组 10 只, 分别 ip 生理盐水 10ml/kg(对照组), 异丙酚 100mg/kg 或氯胺 酮 100 mg/kg。用分光光度法测定大脑皮层 、海马和脑干突触体 Na⁺, K⁺-ATP 酶活性。结果 与对照组比较, 异丙酚明显抑制大脑皮层 、 海马和脑干突触体 Na⁺, K⁺-ATP 酶活性 (P<0.01), 氯胺酮明显降低大脑皮层和脑干突触体 Na⁺, K⁺-ATP 酶活性(P<0.01), 但对海 马突触体 Na⁺, K⁺-ATP 酶活性无明显影响(P<0.01)。结论 异丙酚、氯胺酮的全身麻醉作 用可能与其抑制脑突触体 Na⁺, K⁺-ATP 酶活性有关。

Effects of propofol and ketamine on Na+, K+-ATPase activity of synaptosomes in rat brain in vivo

Aim To investigate the effects of propofol and ketamine on Na⁺, K⁺-ATPase activity in rat brain synaptosomes.Methods Thirty SD rats were divided randomly into three groups. The aminals were given intraperitoneally (ip)normal saline 10ml/kg (control group), propofol 100mg/kg or ketamine 100mg/kg, respectively. Na⁺, K⁺-ATPase activity of synaptosmes was assassed by spectrophotometric analysis.Results In the propofol group Na⁺, K⁺-ATPase activities of cerebrocortical, hippocampal and brain stem synaptosomes were significantly inhibited as compared with those in the control group (P<0.01). In the ke-tamine group, Na⁺, K⁺-ATPase activities of cerebrocortical and brain stem synaptosomes were significantly lower than those in the control group (P<0.01). Conclusions The general anesthesia effects of propofol and ketamine may be related to the inhibition of Na⁺, K⁺-ATPase activity of brain synaptosomes in rats.