| 共振瑞利散射测定痕量甲胎蛋白研究 |
| |
| 引用本文： | 干宁,候琳熙,李天华,徐伟民,周雄.共振瑞利散射测定痕量甲胎蛋白研究[J].应用化工,2007,36(3):224-226. |
| |
| 作者姓名： | 干宁 候琳熙 李天华 徐伟民 周雄 |
| |
| 作者单位： | 宁波大学,新型功能材料及其制备科学国家重点实验室,材料与化工学院,浙江,宁波,315211 |
| |
| 基金项目： | 浙江省科技攻关项目;浙江省宁波市自然科学基金 |
| |
| 摘 要： | 磷钼杂多酸与甲胎蛋白(AFP)结合,会引起共振瑞利散射(RRS),最大RRS峰均位于480 nm,在一定浓度范围内,AFP浓度与散射强度成正比。本文对该反应体系的适宜反应条件、主要影响因素、散射强度与AFP浓度的关系、方法的灵敏度等进行了比较研究。发现不同的杂多酸对于甲胎蛋白的检出限(3σ)在5.2-78 ng/mL之间,其中以磷锑钼酸体系灵敏度最高,对于甲胎蛋白(AFP)的检出限为2.5 ng/mL。该方法具有操作简便、灵敏度高、线性范围宽等优点。考察了共存物质的干扰影响,获得了满意的结果。
|
| 关 键 词： | 共振瑞利散射 AFP 磷钼杂多酸 |
| 文章编号： | 1671-3206(2007)03-0224-03 |
| 修稿时间： | 2006-12-20 |
Determination of AFP in trace by Resonance Rayleigh scattering spectra of phosphato-molybdate heteropoly acid |
| |
| GAN Ning,HOU Lin-xi,LI Tian-hua,XU Wei-min,ZHOU Xiong.Determination of AFP in trace by Resonance Rayleigh scattering spectra of phosphato-molybdate heteropoly acid[J].Applied chemical industry,2007,36(3):224-226. |
| |
| Authors: | GAN Ning HOU Lin-xi LI Tian-hua XU Wei-min ZHOU Xiong |
| |
| Affiliation: | The State Key Laboratory Base of Novel Functional Materials and Preparation Science,Ningbo University,Ningbo 315211 ,China |
| |
| Abstract: | When protein reacts with some large inorganic acid anions such as molybdate to form new products,a maximum resonance scattering(RS) peak at 480 nm for the system of protein-phosphomolybdic acid(PMA) was observed,the optimum conditions,effective factors,the relation between the scattering intensities and the concentration of protein,and the sensitivity of the method have been investigated.The detect limit of different protein-phosphomolybdic acid varies from 5.2 to 78 ng/mL.The sensitivity of molybdate-protein system is the highest,and the detect limit of AFP is 2.5 ng/mL.The method has advantages of easy operation,high sensitivity and wide determination range.Proteins in synthetic samples have been determined by the method with satisfactory results. |
| |
| Keywords: | AFP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
