Simultaneous enzymatic saccharification and ABE fermentation using pretreated oil palm empty fruit bunch as substrate to produce butanol and hydrogen as biofuel |
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| Affiliation: | 1. School of Materials Science and Engineering, Dalian University of Technology, Dalian 116023, China;2. Key Laboratory for Solar Energy Photovoltaic System of Liaoning Province, Dalian 116023, China;3. Department of Physics, Balochistan University of Information Technology, Engineering & Management Sciences, Quetta, Pakistan;4. Qingdao Longsun Silicon Technology Ltd., Qingdao 266000, China;5. Carbon Research Laboratory, Liaoning Key Lab for Energy Materials and Chemical Engineering, State Key Lab of Fine Chemicals, Dalian University of Technology, Dalian 116024, China;1. Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia;2. School of Biosciences and Biotechnology, Faculty of Sciences and Technology, National University of Malaysia (Universiti Kebangsaan Malaysia), 43600 Bangi, Selangor, Malaysia;3. School of Bioprocess Engineering, Universiti Malaysia Perlis, Kompleks Pusat Pengajian Jejawi 3, 02600 Arau, Perlis, Malaysia;1. Department of Biotechnology, University of Chemistry and Technology, Prague, Technicka 5, 16628, Czech Republic;2. Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China |
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| Abstract: | Simultaneous saccharification and acetone–ethanol–butanol (ABE) fermentation was conducted in order to reduce the number of steps involved in the conversion of lignocellulosic biomass into butanol. Enzymatic saccharification of pretreated oil palm empty fruit bunch (OPEFB) by cellulase produced 31.58 g/l of fermentable sugar. This saccharification was conducted at conditions similar to the conditions required for ABE fermentation. The simultaneous process by Clostridium acetobutylicum ATCC 824 produced 4.45 g/l of ABE with butanol concentration of 2.75 g/l. The butanol yield of 0.11 g/g and ABE yield of 0.18 g/g were obtained from this simultaneous process as compared to the two-step process (0.10 g/g of butanol yield and 0.14 g/g of ABE yield). In addition, the simultaneous process also produced higher cumulative hydrogen (282.42 ml) than to the two-step process (222.02 ml) after 96 h of fermentation time. This study suggested that the simultaneous process has the potential to be implemented for the integrated production of butanol and hydrogen from lignocellulosic biomass. |
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| Keywords: | Biobutanol Biohydrogen Saccharification Lignocellulosic biomass Simultaneous saccharification fermentation ABE" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" acetone–butanol–ethanol DCW" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" dry cell weight DNS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" dinitrosalicylic acid GHG" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" greenhouse gasses OD" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" optical density OPEFB" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" oil palm empty fruit bunch RCM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" Reinforced Clostridial Medium |
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