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皇竹草稀硫酸预处理研究
引用本文:陈尚钘,方楷,王宗德,范国荣,王鹏.皇竹草稀硫酸预处理研究[J].生物质化学工程,2014,48(6):30-35.
作者姓名:陈尚钘  方楷  王宗德  范国荣  王鹏
作者单位:1. 江西农业大学 林学院, 江西 南昌 330045; 2. 江西农业大学 江西省竹子种质资源与利用重点实验室, 江西 南昌 330045
基金项目:国家林业公益性行业科研专项(201204801);高等学校博士学科点专项科研基金(20103603120004);江西省科技支撑计划项目
摘    要:研究了中低温稀酸预处理对皇竹草化学组成变化、纤维素酶水解得率与总糖得率的影响,并采用扫描电镜(SEM)对皇竹草纤维结构变化进行了分析.结果表明,随着硫酸浓度的增大、温度的升高和时间的延长,半纤维素含量大幅度降低,且预处理后纤维素酶水解得率也逐渐增大.较好的预处理条件为100 g皇竹草原料,在固液比1:5(g:mL)条件下,用质量分数4.0%硫酸在温度110 ℃下,经过8 h预处理后,纤维素保留率为87.48%,半纤维素水解率为93.68%,所得固体渣经纤维素酶水解72 h后得率为86.3%(纤维素酶用量40 FPIU/g,以纤维素质量计),100 g原料可得到总糖量为54.53 g.预处理后皇竹草纤维表面和细胞壁受到破坏,表面积增大,有利于纤维素酶水解作用的进行.

关 键 词:皇竹草  稀硫酸预处理  半纤维素水解  纤维素酶水解  电镜扫描  
收稿时间:2014-06-22

Pretreatment of Pennisetum Grass with Dilute Sulfuric Acid
CHEN Shang-xing,FANG Kai,WANG Zong-de,FAN Guo-rong,WANG Peng.Pretreatment of Pennisetum Grass with Dilute Sulfuric Acid[J].Biomass Chemical Engineering,2014,48(6):30-35.
Authors:CHEN Shang-xing  FANG Kai  WANG Zong-de  FAN Guo-rong  WANG Peng
Affiliation:1. College of Forestry, Jiangxi Agricultural University, Nanchang 330045, China; 2. Jiangxi Provincial Key Laboratory for Bamboo Germplasm Resources and Utilization, Jiangxi Agriculture University, Nanchang 330045, China
Abstract:The effects of dilute acid pretreatment conditions of Pennisetum grass on the composition,celluloses enzymatic hydrolysis yield, and the yield of total sugars in liquids were investigated at medium-low temperature. And the fiber structures of pretreated residues were studied with scanning electron microscope (SEM).With the increase of sulfuric concentration,temperature,and retention time,the hemicelluloses content declined considerably,and the yield of enzymatic hydrolysis increased gradually.The recovery of cellulose was 87.48%,the hemicelluloses hydrolysis rate was 93.68%,the yield of enzymatic hydrolysis was 86.3%,and the total sugars yield was 54.53 g/100 g after the pretreatment when the pretreatment conditions were sulfuric acid concentration 4.0%,temperature 110 ℃,residence time 8 h,and cellulase dosage 40 FPIU/g cellulose.The results indicated that the fiber surface and cell wall of Pennisetum grass were broken after acid pretreatment,thus the specific surface area and pore volume increased,and it was beneficial to the enzymatic hydrolysis.
Keywords:Pennisetum grass(Pennisetum hydridum)  dilute sulfuric acid pretreatment  hemicellulose hydrolysis  enzymatic hydrolysis  scanning electron microscope(SEM)
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