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| 离子辐照对生防菌BJ1的诱变研究及菌种鉴定 | |
| 引用本文： | 马爽,李文建,王菊芳,陆栋,刘敬. 离子辐照对生防菌BJ1的诱变研究及菌种鉴定[J]. 辐射研究与辐射工艺学报, 2009, 27(2) |
| 作者姓名： | 马爽 李文建 王菊芳 陆栋 刘敬 |
| 作者单位： | 1. 中国科学院近代物理研究所,兰州,730000;中国科学院研究生院,北京,100039 2. 中国科学院近代物理研究所,兰州,730000 |
| 基金项目： | 中国科学院西部之光资助项目 |
| 摘 要： | 为了提高生防菌BJl的抑菌能力,优化辐照诱变的物理参数,获得生防效果更好的高效菌种,利用12C6+对生防菌BJ1进行不同剂量离子辐照处理.结果表明,采用100 Gy剂量辐照的生防菌BJ1的存活率最高,并且在0～100 Gy之间,存活率先降后升,其存活曲线呈"鞍型";从诱变效果看,经400 Gy辐照诱变获得的生防菌BJ1的抑菌效果有明显提高;综合存活和抑菌效果考虑,离子辐照诱变生防菌BJ1的最适剂量为200～400 Gy.通过16S rDNA序列分析,将辐照诱变获得的BJ1菌株定为枯草芽孢杆菌. |
| 关 键 词： | 离子辐照 生防菌BJ1 诱变 菌种鉴定 |
Studies on the effects of ~(12)C~(6+) ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1 |
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| MA Shuang,LI Wenjian,WANG Jufang,LU Dong,LIU Jing. Studies on the effects of ~(12)C~(6+) ion beam irradiation to biocontrol bacterium-BJ1 and identification of BJ1[J]. Journal of Radiation Research and Radiation Processing, 2009, 27(2) | |
| Authors: | MA Shuang LI Wenjian WANG Jufang LU Dong LIU Jing |
| Affiliation: | Institute of Modern Physics;Chinese Academy of Sciences;Lanzhou 730000;China;Graduate School of Chinese Academy of Sciences;Beijing 100039;China |
| Abstract: | Biocontrol bacterium BJ1 was irradiated with different doses of 12C6+ ion beam in order to improve the antagonistic ability, to optimize the parameters of ion beam irradiation and to obtain high effect of biocontrol strains. The results show that the survival rate of BJ1 irradiated with 100 Gy reaches the highest and the survival curve of BJ1 presents a tendency of going down first and then increasing, which takes a shape of "saddle" during 0 to100 Gy. In view of the effect of mutation induction, BJ1 irradi... |
| Keywords: | Ion beam irradiation Biocontrol bacterium-BJ1 Mutation Identification |
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