固相萃取-流动注射化学发光法测定动物肝脏中的盐酸二氟沙星

碱性条件下,基于盐酸二氟沙星对鲁米诺-过氧化氢化学发光反应体系的抑制作用,样品经固相萃取柱处理后用该体系测定,建立快速分析猪肝、鸡肝中盐酸二氟沙星的流动注射化学发光新方法。结果表明:盐酸二氟沙星质量浓度在1.00×10-5～1.00×10-9mg/mL范围内,质量浓度的负对数与体系的相对化学发光强度呈良好线性关系,方法检出限为4.07×10-9mg/mL,相对标准偏差(RSD)为0.93%(n=11)。该方法用于动物肝脏中盐酸二氟沙星残留的检测,结果令人满意。

Determination of Difloxacin Hydrochloride Residue in Animal Liver by Solid Phase Extraction and Flow Injection Chemiluminescence

A new quick method for the determination of difloxacin hydrochloride residue in pork and chicken livers using solid phase extraction followed by flow injection chemiluminescence analysis was presented based on the inhibition of chemiluminescence generation from the reaction between luminol and hydrogen peroxide by difloxacin hydrochloride.The results showed that an excellent linear relationship between chemiluminescence intensity and negative logarithm of difloxacin hydrochloride concentration was observed in the range of 1.00 × 10-5-1.00×10-9 mg/mL.The limit of detection for difloxacin hydrochloride was 4.07 × 10-9 mg/mL.The precision was 0.93%(relative standard deviation,RSD) for eleven replicate determinations.Therefore,this method can be used for the determination of difloxacin hydrochloride in animal liver with satisfactory results.