盖玻片在培养细胞免疫电子显微术中的应用

体外培养的细胞不仅可用于研究其形态和机能的变化，而且可进行不同药物学作用时细胞内抗原成分的定位检测及细胞特征的研究。通常盖玻片放入培养皿中作为培养细胞的支持物，细胞在盖玻片上长成单层，可立即固定、染色，进行光学显微镜的观察。在实验中我们发现，加盖玻片的单层细胞培养法也可以直接应用于免疫电镜的包埋前和包埋后染色方法。该技术具有以下优点。(1)使用盖玻片可以避免免疫电镜标本制作过程中，为了搜集细胞而进行的数次刮取、离心。免疫反应及后固定、脱水、浸透、包埋均在盖玻片上进行，使培养细胞的损伤降低至最小程度。(2)如果在直径3．5cm的培养皿中进行免疫过氧化物酶反应大约需要至少200μl抗体，而使用盖玻片法，每张盖玻片则仅需要10μl抗体，大大节约了免疫反应的抗体量，既简便又经济。(3)盖玻片法使包埋后培养细胞呈单层位于包埋块的表面，在每一张超薄切片中都能观察到很多细胞。

Application of cover slip to immunoelectron microscopy of culture cell

We propose an application of coverslip to immunoelectron microscopy. In our experiment, it is found that culture cells on cover slip could be applied directly to immunoelectron microscopy with pre-embedding and post-embedding methods. By these methods,(1) we can save amounts of antibodies for immunoreaction; (2) we can observe culture cells from en face view, therefore can observe more cells in each section than those with other methods; (3) by using cover slip, we can avoid handling culture cells with several times of centrifuging, scraping and can decrease damage of the culture cells to the smallest degree. These techniques are simple and useful not only in immunocytochemistry, but also in conventional morphological analysis for most culture cells.