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Effect of Ultrafiltration on Anthocyanin and Flavonol Content of Black Currant Juice (Ribes nigrum L.)
Authors:Nora Pap  Mika Mahosenaho  Eva Pongrácz  Helmi Mikkonen  Mari Jaakkola  Vesa Virtanen  Liisa Myllykoski  Zsuzsanna Horváth-Hovorka  Cecília Hodúr  Gyula Vatai  Riitta L Keiski
Affiliation:1. Department of Process and Environmental Engineering, Mass and Heat Transfer Process Laboratory, University of Oulu, 90014, P.O.Box?4300, Oulu, Finland
2. Laboratory of Biotechnology, Kajaani University Consortium, University of Oulu, 88600, Salmelantie 43, Sotkamo, Finland
3. Thule Institute, NorTech Oulu, University of Oulu, 90014, P.O.Box?3700, Oulu, Finland
4. Department of Process and Environmental Engineering, Bioprocess Engineering Laboratory, University of Oulu, 90014, P.O.Box?4300, Oulu, Finland
5. Faculty of Engineering, Department of Process and Mechanical Engineering, University of Szeged, 6725, Szeged, Moszkvai krt. 5-7, Hungary
6. Department of Food Engineering, Corvinus University of Budapest, 1118, Budapest, M??nesi ??t 44., Hungary
Abstract:This paper evaluates the efficiency of ultrafiltration and the effects of processing on the total anthocyanin and flavonol contents of black currant juice at chosen operational conditions. Ultrafiltration of black currant juices was carried out using Biomax 100?kDa polyethersulfone membrane. Ultrafiltration was used to process the juice prior concentration by reverse osmosis; with the aim to enhance the efficiency of the concentration process in terms of permeate flux. To avoid the fouling of the membrane, the juices were depectinized with Panzym Super E liquid enzyme preparation. The ultrafiltration was carried out at a transmembrane pressure of 2?bars and the operating temperature of 25?°C. The effect of processing on the valuable anthocyanin and flavonol content of the juices was evaluated based on the results of high-performance liquid chromatography analyses. The article includes detailed analyses of anthocyanin and flavonol compounds of the enzyme treated and ultrafiltered juice as compared with the original juice. The results indicate that, due to the enzymatic treatment, the valuable compound content of the juice increases. However, the ultrafiltration process resulted in a significant loss of a valuable content; 54% of total flavonol and 50% of total anthocyanins maintained in ultrafiltered juice when compared to the feed samples.
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