缺氧复氧诱导的大鼠海马神经元凋亡和一氧化氮合酶表达变化及银杏叶提取物的作用

目的: 观察银杏叶提取物(EGb) 及其活性成份银杏总内酯(Gin) 对缺氧再复氧诱导的大鼠海马神经元凋亡及一氧化氮合酶(nitric oxide synthase,NOS) 表达影响。方法: 选用胎龄15 d 的SD 胎鼠的大脑海马细胞进行原代培养, 建立缺氧再复氧海马神经元损伤模型。实验分以下几组:正常组、缺氧复氧组、EGb 组、Gin 组、阳性对照组(MK-801 组)。应用TUNEL 原位末端标记法定量分析细胞凋亡以及NADPH-d 组织化学染色法观察细胞NOS 的表达。结果: (1) TUNEL 免疫细胞化学染色结果显示, 预先加入EGb 和Gin 的药物组神经元的凋亡率明显低于缺氧复氧组;(2) NADPH-d 组织化学染色结果显示,预先加入EGb 和Gin 的药物组抑制神经元NOS 表达,NADPH 阳性细胞减少。结论: EGb 及其活性成份Gin 对缺氧再复氧损伤的海马神经元具有保护作用, 可部分拮抗缺氧再复氧条件下体外培养的海马神经元的凋亡, 抑制NOS 的表达。

Effects of EGb on apoptosis and NOS activity in rat hippocampal neurons induced by hypoxia

AIM: To observe the effects of EGb (Ginkgo biloba extract) and Gin(Ginkgolide) on rat hippocampal neurons induced by H/R(Hypoxia/Reoxigen) with regards of apoptosis and NOS activity.METHODS: Primarily cultured E 15 day-fetal rat hippocampal neurons were used to establish H/R injury model including five groups:Normal group, H/R group, Drug group (EGb group and Gin group) and Positive control group (MK-801 group).The neuronal apoptosis were measured by TUNEL staining.The NOS activity was determined by NADPH-d histochemical analysis.RESULTS: The apoptosis cell numbers of EGb and Gin groups were lower than those of H/R group.The preincubation of EGb and Gin decreased NADPH-positive cells.CONCLUSION: EGb and Gin protects rat hippocampal neurons induced by H/R from apoptosis and suppressed NOS activity.