| 一氧化氮诱导 PC12 细胞凋亡及芍药苷的保护作用 |
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| 引用本文： | 孙蓉,武栋栋,刘国卿.一氧化氮诱导 PC12 细胞凋亡及芍药苷的保护作用[J].金属学报,2005,10(11):1266-1274. |
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| 作者姓名： | 孙蓉 武栋栋 刘国卿 |
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| 作者单位： | 1.中国药科大学药理教研室,南京 210009,江苏;2.山东省中医药研究院,济南 250014,山东 |
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| 摘 要： | 目的: 探讨一氧化氮诱导PC12细胞凋亡及芍药苷保护作用的可能机制。方法: MTT和乳酸脱氢酶活性测定细胞存活率,DNA凝胶电泳观察DNA的断裂情况,流式细胞仪测定细胞凋亡率、检测线粒体跨膜电位。结果: 500μmol·L-1硝普钠(SNP)可诱导PC12细胞凋亡,细胞线粒体跨膜电位明显下降。预先经过0.1、1和10μmol·L-1等浓度芍药苷处理后,SNP诱导的PC12细胞凋亡明显减少,同时明显减弱一氧化氮对线粒体跨膜电位的影响。结论: 芍药苷可抑制一氧化氮诱导PC12细胞凋亡,其作用机制可能与其稳定细胞线粒体跨膜电位有关。
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| 关 键 词： | 芍药苷 一氧化氮 凋亡 线粒体 PC12细胞 |
| 收稿时间： | 2005-05-18 |
| 修稿时间： | 2005-08-02 |
Nitric oxide-induced PC12 cells apoptosis and protective effect of paeoni-florin |
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| SUN Rong,WU Dong-dong,LIU Guo-qing.Nitric oxide-induced PC12 cells apoptosis and protective effect of paeoni-florin[J].Acta Metallurgica Sinica,2005,10(11):1266-1274. |
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| Authors: | SUN Rong WU Dong-dong LIU Guo-qing |
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| Affiliation: | 1.Department of Pharmacology,China Pharmaceutical University,Nanjing 210009,Jiangsu,China;2.Shandong Research Academy of TCM,Jinan 250014,Shandong,China |
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| Abstract: | AIM: To explore the possible mechanism of nitric oxide-induced PC12 cells apoptosis and the pro-tective effect of paeoniflorin.METHODS: Cell survival was detected by MTT and lactate dehydrogenase detec-tion.DNA fragmentation was observed by gels electro-phoresis.Apoptotic cells and mitochondral transmembrane potentiall were observed by flow cytometry in PC12 cells.RESULTS: 500μmol·L-1of SNPinduced PC12 cells apoptosis.Paeoniflorin decreased nitric oxide induced PC12 cells apoptotic rate and increased mitochondrial transmembrane potential.CONCLUSION: Paeoniflorin can inhibit nitric oxide-induced apoptosis in PC12 cells.The mechanism is related to preventing loss of MMPin PC12 cells. |
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| Keywords: | paeoniflorin nitric oxide apoptosis mitochondrial PC12 cell |
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