新型吡唑啉衍生物合成与抑菌活性实验研究

根据α,β-不饱和醛酮亲核加成反应原理合成化合物L{3-(4-N,N-dimethyl-pyenyl)-5-Anthracen-9-yl]-Pyrazoline}和化合物M3-ferroceny-5-(Anthracen-9-yl)-Pyrazoline]。利用~1H NMR和~(13)C NMR对化合物进行结构表征,通过抑菌实验研究化合物抑菌生物活性。探析新型吡唑啉衍生物对革兰氏阳性菌、革兰氏阴性菌和真菌的抑菌效果,研究其结构变化对活性的影响。结果表明,化合物L和M对典型革兰氏阳性菌和典型革兰氏阴性菌有一定的抑菌效果,对真核细胞型微生物作用不明显。通过比较两者对细菌的作用结果(抑菌圈直径的大小),化合物L的抑菌作用明显强于化合物M,化合物L吡唑啉环连接N、N-二甲基苯环可能增强了抑菌生物活性。

Synthesis of novel pyrazoline derivatives and experimental study on antibacterial activity

Compound L{3-(4-N,N-dimethyl-pyenyl)-5-Anthracen-9-yl]-Pyrazoline} and compound M3-ferroceny-5-(Anthracen-9-yl)-Pyrazoline] were synthesized according to the nucleophilic addition reaction principle of α,β unsatur ated aldehydes and.The compounds were characterized by ¹H NMR and ¹³C NMR,the antibacterial activity of compounds was studied by bacteriostasis experiments.The bacteriostatic effect of new pyrazoline derivatives on gram-positive bacteria,gram-negative bacteria and fungi was studied,and the effects of their structural changes on their activity were investigated.It was proved that compounds L and M had certain bacteriostatic effect on typical gram positive bacteria and typical gram negative bacteria,but no effect on eukaryotic cell microorganism.By comparing to the results of the bacteriostasis(diameter of bacteriostasis),the antibacterial activity of compound L was obviously stronger than that of compound M.Pyrazoline ring in L linking to N and N- two methyl benzene ring might enhance the bacteriostasis activity.