Rapid and specific sensing of tetracycline in food using a novel upconversion aptasensor |
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| Affiliation: | 1. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China;2. Institute of Agricultural Engineering, Jiangsu University, Zhenjiang 212013, PR China;1. Nanotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;2. Research Institute of Sciences and New Technology, Mashhad, Iran;3. Academic Center For Education, Culture and Research (ACECR)-Mashhad Branch, Mashhad, Iran;4. Department of Chemistry, Ferdowsi University of Mashhad, Mashhad, Iran;5. Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;6. Targeted drug delivery Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;1. Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin 300457, China;2. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, China;1. Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, PR China;2. Institute of Food Safety, Chinese Academy of Inspection and Quarantine, Beijing 100123, PR China;1. School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha 410004, China;2. State Key Laboratory of Medical Genetics & School of Life Sciences, Central South University, Changsha 410013, China |
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| Abstract: | Rare earth-doped upconversion nanoparticles (UCNPs) have a promising potential in biodetection due to their unique frequency upconverting capability and high detection sensitivity. This study developed a novel and sensitive aptamer-based UCNPs (NaY0.48Gd0.3F4:Yb0.2, Ho0.02) biosensor for sensing tetracycline (TET) in foodstuffs. The aptamer-magnetic nanoparticles (aptamer-MNPs) and the complementary DNA-UCNPs (cDNA-UCNPs) conjugates were prepared and used as the capture and signal probes, respectively. They formed the complex of MNPs-aptamer-cDNA-UCNPs with complete binding. With TET addition, the aptamer preferentially bound with TET and caused the dissociation of some cDNA; and the liberation of some cDNA-UCNPs. This led to a decreased fluorescent signal on the surface of MNPs. Under the optimal conditions, a wide linear detection range from 0.01 to 100 ng/mL was achieved, with a limit of detection of 0.0062 ng/mL, for detection of TET. The proposed method was successfully applied to measure TET in contaminated samples. Results showed that the proposed UCNPs biosensor offers an efficient, specific, and simple approach for the detection of TET in food, and has high potential for food safety and quality control. |
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| Keywords: | Tetracycline Upconversion nanoparticles Aptasensor Milk Pork |
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